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In-Vitro Dissolution Dynamic Nuclear Polarization for Sensitivity Enhancement of NMR with Biological Molecules

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Protein NMR

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1688))

Abstract

Dissolution dynamic nuclear polarization (D-DNP) is a technique to prepare hyperpolarized nuclear spin states, yielding a signal enhancement of several orders of magnitude for liquid-state NMR. Here, we describe experimental procedures for the application of D-DNP in high-resolution NMR of biochemical compounds, to determine the time evolution of biochemical processes and intermolecular interactions.

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Authors and Affiliations

  1. Chemistry Department, Texas A&M University, 3255 TAMU, College Station, TX, 77843, USA

    Yaewon Kim, Yunyi Wang & Christian Hilty

  2. Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, MD, 20892-0520, USA

    Hsueh-Ying Chen

Authors
  1. Yaewon Kim
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  2. Yunyi Wang
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  3. Hsueh-Ying Chen
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  4. Christian Hilty
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Corresponding author

Correspondence to Christian Hilty .

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Editors and Affiliations

  1. Department of Chemistry and Biochemistry, The City College of New York, New York, New York, USA

    Ranajeet Ghose

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Kim, Y., Wang, Y., Chen, HY., Hilty, C. (2018). In-Vitro Dissolution Dynamic Nuclear Polarization for Sensitivity Enhancement of NMR with Biological Molecules. In: Ghose, R. (eds) Protein NMR. Methods in Molecular Biology, vol 1688. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7386-6_8

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  • DOI: https://doi.org/10.1007/978-1-4939-7386-6_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7385-9

  • Online ISBN: 978-1-4939-7386-6

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