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Probing the Atomic Structure of Transient Protein Contacts by Paramagnetic Relaxation Enhancement Solution NMR

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Protein NMR

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1688))

Abstract

Important biological processes, including enzyme catalysis, signaling, and protein folding, proceed through lowly populated (<5%) states that elude structural characterization by conventional techniques. Here, we describe the steps required for visualization of these sparsely populated conformations and transient protein-protein interactions using paramagnetic relaxation enhancement solution NMR. We describe experimental design, sample preparation, data acquisition and processing, and the basics of data analysis of structural ensembles.

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Acknowledgments

Research reported in this publication was supported in part by the National Institute Of General Medical Sciences (NIGMS) of the National Institutes of Health (NIH) under Award Number R01GM118530 (to N.L.F) and by startup funding from Iowa State University (to V.V.).

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Authors and Affiliations

  1. Department of Chemistry, Iowa State University, 0219 Hach Hall, 2438 Pammel Drive, Ames, IA, 50011, USA

    Vincenzo Venditti

  2. Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, 0219 Hach Hall, 2438 Pammel Drive, Ames, IA, 50011, USA

    Vincenzo Venditti

  3. Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Box G-E, 171 Meeting Street, Providence, RI, 02912, USA

    Nicolas L. Fawzi

Authors
  1. Vincenzo Venditti
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  2. Nicolas L. Fawzi
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Corresponding authors

Correspondence to Vincenzo Venditti or Nicolas L. Fawzi .

Editor information

Editors and Affiliations

  1. Department of Chemistry and Biochemistry, The City College of New York, New York, New York, USA

    Ranajeet Ghose

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Venditti, V., Fawzi, N.L. (2018). Probing the Atomic Structure of Transient Protein Contacts by Paramagnetic Relaxation Enhancement Solution NMR. In: Ghose, R. (eds) Protein NMR. Methods in Molecular Biology, vol 1688. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7386-6_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7386-6_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7385-9

  • Online ISBN: 978-1-4939-7386-6

  • eBook Packages: Springer Protocols

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