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Analysis of Lipids and Lipid Rafts in Borrelia

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Borrelia burgdorferi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1690))

Abstract

Lipid rafts are membrane microdomains that are involved in cellular processes such as protein trafficking and signaling processes, and which play a fundamental role in membrane fluidity and budding. The lipid composition of the membrane and the biochemical characteristics of the lipids found within rafts define the ability of cells to form microdomains and compartmentalize the membrane. In this chapter, we describe the biophysical, biochemical, and molecular approaches used to define and characterize lipid rafts in the Lyme disease agent, Borrelia burgdorferi.

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Acknowledgments

This work was supported by NIH grants 1R21AI125806 to AT, GM 099892 to EL and RO1-AI-027044 to JLB.

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Authors and Affiliations

  1. Department of Entomology, Rutgers University, New Brunswick, NJ, USA

    Alvaro Toledo

  2. Department of Molecular Genetics and Microbiology, Stony Brook University, Health Science Center, Level 4, Stony Brook, NY, 11794 8434, USA

    Jorge L. Benach

  3. Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA

    Zhen Huang & Erwin London

Authors
  1. Alvaro Toledo
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  2. Zhen Huang
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  3. Jorge L. Benach
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  4. Erwin London
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Corresponding author

Correspondence to Alvaro Toledo .

Editor information

Editors and Affiliations

  1. Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA

    Utpal Pal

  2. Department of Microbiology, Ondokuz Mayis University, Samsun, Turkey

    Ozlem Buyuktanir

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Toledo, A., Huang, Z., Benach, J.L., London, E. (2018). Analysis of Lipids and Lipid Rafts in Borrelia . In: Pal, U., Buyuktanir, O. (eds) Borrelia burgdorferi. Methods in Molecular Biology, vol 1690. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7383-5_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7383-5_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7382-8

  • Online ISBN: 978-1-4939-7383-5

  • eBook Packages: Springer Protocols

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