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Characterization of Borrelia burgdorferi Binding to Mammalian Cells and Extracellular Matrix

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Borrelia burgdorferi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1690))

Abstract

Lyme disease Borreliae produces outer surface adhesins to confer bacterial attachment to the extracellular matrix (ECM) components on the surface of mammalian cells. Here, we describe protocols to characterize the activity and specificity of these adhesins by flow cytometry or measurement of the binding of radiolabeled spirochetes to immobilized ECM or mammalian cells.

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Acknowledgment

We thank Ashley Marcinkiewicz, Michael Pereira, and Beau Wager for critical reading of the manuscript. This work was supported by R01 AI118799 (to J.M.L.) and Wadsworth Center Start-Up Grant (to Y.L.).

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Authors and Affiliations

  1. Division of Infectious Disease, Wadsworth Center, New York State Department of Health, 120 New Scotland Ave., Albany, NY, 12208, USA

    Yi-Pin Lin

  2. Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA, 02111, USA

    John M. Leong

Authors
  1. Yi-Pin Lin
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  2. John M. Leong
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Corresponding author

Correspondence to Yi-Pin Lin .

Editor information

Editors and Affiliations

  1. Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA

    Utpal Pal

  2. Department of Microbiology, Ondokuz Mayis University, Samsun, Turkey

    Ozlem Buyuktanir

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Lin, YP., Leong, J.M. (2018). Characterization of Borrelia burgdorferi Binding to Mammalian Cells and Extracellular Matrix. In: Pal, U., Buyuktanir, O. (eds) Borrelia burgdorferi. Methods in Molecular Biology, vol 1690. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7383-5_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7383-5_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7382-8

  • Online ISBN: 978-1-4939-7383-5

  • eBook Packages: Springer Protocols

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