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Two Photon Intravital Microscopy of Lyme Borrelia in Mice

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Book cover Borrelia burgdorferi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1690))

Abstract

Two-photon intravital microscopy is a powerful tool that allows visualization of cells in intact tissues in a live animal in real time. In recent years, this advanced technology has been applied to understand pathogen-host interactions using fluorescently labeled bacteria. In particular, infectious fluorescent transformants of the Lyme disease spirochete Borrelia burgdorferi, an Ixodes tick-transmitted pathogen, have been imaged by two-photon intravital microscopy to study bacterial motility and interactions of the pathogen with feeding ticks and host tissues. Here, we describe the techniques and equipment used to image mammalian-adapted spirochetes in the skin of living mice in vivo and in joints ex vivo using two-photon intravital microscopy.

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Authors and Affiliations

  1. Department of Internal Medicine/Section of Rheumatology, Yale University School of Medicine, New Haven, CT, 06520, USA

    Alexia A. Belperron Ph.D., Jialing Mao & Linda K. Bockenstedt

Authors
  1. Alexia A. Belperron Ph.D.
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  2. Jialing Mao
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  3. Linda K. Bockenstedt
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Corresponding author

Correspondence to Alexia A. Belperron Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA

    Utpal Pal

  2. Department of Microbiology, Ondokuz Mayis University, Samsun, Turkey

    Ozlem Buyuktanir

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Belperron, A.A., Mao, J., Bockenstedt, L.K. (2018). Two Photon Intravital Microscopy of Lyme Borrelia in Mice. In: Pal, U., Buyuktanir, O. (eds) Borrelia burgdorferi. Methods in Molecular Biology, vol 1690. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7383-5_20

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  • DOI: https://doi.org/10.1007/978-1-4939-7383-5_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7382-8

  • Online ISBN: 978-1-4939-7383-5

  • eBook Packages: Springer Protocols

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