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Selection of Borrelia burgdorferi Promoter Sequences Active During Mammalian Infection Using In Vivo Expression Technology

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Borrelia burgdorferi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1690))

Abstract

In vivo expression technology (IVET) has been applied to a variety of organisms to identify active promoters in specific environments or growth conditions of interest. Here, we describe modifications to employ this genome-wide screening method for Borrelia burgdorferi, the Lyme disease spirochete, during an active murine infection. Utilization of this technique provides valuable insights into the B. burgdorferi transcriptome during infection, despite the low bacterial numbers in the mammalian host environment.

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Acknowledgment

Thank you to Dr. Andrew Camilli, Dr. Patti Rosa and members of the Rosa lab for initial guidance on development of IVET for B. burgdorferi. Thank you to members of the Jewett lab for all of their hard work on this project. In particular, we recognize the invaluable contributions of Angelika Linowski and Dr. Sunny Jain. This work was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under award numbers K22AI081730 and R01AI099094 to M.W.J.

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Authors and Affiliations

  1. Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, 6900 Lake Nona Blvd., Orlando, FL, 32827, USA

    Philip P. Adams & Mollie W. Jewett

Authors
  1. Philip P. Adams
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  2. Mollie W. Jewett
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Correspondence to Mollie W. Jewett .

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Editors and Affiliations

  1. Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA

    Utpal Pal

  2. Department of Microbiology, Ondokuz Mayis University, Samsun, Turkey

    Ozlem Buyuktanir

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Adams, P.P., Jewett, M.W. (2018). Selection of Borrelia burgdorferi Promoter Sequences Active During Mammalian Infection Using In Vivo Expression Technology. In: Pal, U., Buyuktanir, O. (eds) Borrelia burgdorferi. Methods in Molecular Biology, vol 1690. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7383-5_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7383-5_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7382-8

  • Online ISBN: 978-1-4939-7383-5

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