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Probing the Pathogenesis of Duchenne Muscular Dystrophy Using Mouse Models

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1687)

Abstract

Investigations using mouse models have provided seminal insights into the pathogenesis of Duchenne muscular dystrophy and the development of novel therapeutics. Several important methods have been considered standard-in-the-field for analyses of skeletal muscle weakness, strength, endurance, and histopathology, as well as responses to therapeutics such as glucocorticoids, disease modifying drugs which are part of the current standard of care for patients with this disease. Here we describe optimized genetic, genomic, and physiologic assays to probe dystrophic pathobiology in the mdx mouse and related strains.

Key words

mdx Duchenne Muscular dystrophy Exercise Glucocorticoids Skeletal muscle 

Notes

Acknowledgments

This work was supported by NIH grants R01-DK093821 (SMH), R01-HL127240 (SMH), T32-HL105338 (AM-N) and an Individual Biomedical Research Award from The Hartwell Foundation (SMH).

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Alexander Morrison-Nozik
    • 1
  • Saptarsi M. Haldar
    • 2
    • 3
  1. 1.Ohio University Heritage College of Osteopathic Medicine at Cleveland ClinicClevelandUSA
  2. 2.Gladstone InstitutesSan FranciscoUSA
  3. 3.Department of MedicineUniversity of California San FranciscoSan FranciscoUSA

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