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Duchenne Muscular Dystrophy pp 75–89Cite as

System Biology Approach: Gene Network Analysis for Muscular Dystrophy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1687))

Abstract

Phenotypic changes at different organization levels from cell to entire organism are associated to changes in the pattern of gene expression. These changes involve the entire genome expression pattern and heavily rely upon correlation patterns among genes. The classical approach used to analyze gene expression data builds upon the application of supervised statistical techniques to detect genes differentially expressed among two or more phenotypes (e.g., normal vs. disease). The use of an a posteriori, unsupervised approach based on principal component analysis (PCA) and the subsequent construction of gene correlation networks can shed a light on unexpected behaviour of gene regulation system while maintaining a more naturalistic view on the studied system.

In this chapter we applied an unsupervised method to discriminate DMD patient and controls. The genes having the highest absolute scores in the discrimination between the groups were then analyzed in terms of gene expression networks, on the basis of their mutual correlation in the two groups. The correlation network structures suggest two different modes of gene regulation in the two groups, reminiscent of important aspects of DMD pathogenesis.

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Authors and Affiliations

  1. Department of Cardiovascular, Dysmetabolic and Aging-associated Diseases, Italian National Institute of Health, Viale Regina Elena 299, 00161, Rome, Italy

    Federica Censi, Giovanni Calcagnini & Eugenio Mattei

  2. Department of Environment and Health, Italian National Institute of Health, Viale Regina Elena 299, 00161, Rome, Italy

    Alessandro Giuliani

Authors
  1. Federica Censi
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  2. Giovanni Calcagnini
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  3. Eugenio Mattei
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  4. Alessandro Giuliani
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Corresponding author

Correspondence to Federica Censi .

Editor information

Editors and Affiliations

  1. Istituto di Anatomia Umana e Biologia Cellulare, Università Cattolica del Sacro Cuore, Roma, Italy

    Camilla Bernardini

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Censi, F., Calcagnini, G., Mattei, E., Giuliani, A. (2018). System Biology Approach: Gene Network Analysis for Muscular Dystrophy. In: Bernardini, C. (eds) Duchenne Muscular Dystrophy. Methods in Molecular Biology, vol 1687. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7374-3_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7374-3_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7373-6

  • Online ISBN: 978-1-4939-7374-3

  • eBook Packages: Springer Protocols

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