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Proton Nuclear Magnetic Resonance (1H NMR) Spectroscopy-Based Analysis of Lipid Components in Serum/Plasma of Patients with Duchenne Muscular Dystrophy (DMD)

  • Niraj Kumar Srivastava
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1687)

Abstract

Proton nuclear magnetic resonance spectroscopy (1H NMR) is a useful tool for the analysis of lipid components in biofluids such as serum/plasma. Such tool is applied for the analysis of lipid components in serum/plasma of patients with Duchenne muscular dystrophy (DMD). The practical approach of sampling, storage, lipid extraction procedure, sample preparation before performing the 1H NMR spectroscopy experiments is presented. All the experimental parameters of NMR spectroscopy are also described. Details of the assignments of lipid components (qualitative analysis) and quantification of particular lipid components (quantitative analysis) are explained.

Key words

Triglycerides Phospholipids Cholesterol Muscular dystrophy DMD NMR spectroscopy Metabolomics 

Abbreviations

CHOL

Free cholesterol

CHOLest

Esterified cholesterol

DMD

Duchenne Muscular Dystrophy

NMR

Nuclear magnetic resonance

PL

Phospholipids

TG

Triglycerides

TSP

[3-(trimethylsilyl) propionic-2, 2, 3, 3-d4 acid, sodium salt]

Notes

Acknowledgment

The author wishes to thank University Grant Commission, Government of India, for their generous financial support [Grant number: No.F.4-2/2006 (BSR)/13-194/2008 (BSR)].

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.School of Life Sciences (SLS)Jawaharlal Nehru University (JNU)New DelhiUSA

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