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Proton Nuclear Magnetic Resonance (1H NMR) Spectroscopy-Based Analysis of Lipid Components in Serum/Plasma of Patients with Duchenne Muscular Dystrophy (DMD)

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Duchenne Muscular Dystrophy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1687))

Abstract

Proton nuclear magnetic resonance spectroscopy (1H NMR) is a useful tool for the analysis of lipid components in biofluids such as serum/plasma. Such tool is applied for the analysis of lipid components in serum/plasma of patients with Duchenne muscular dystrophy (DMD). The practical approach of sampling, storage, lipid extraction procedure, sample preparation before performing the 1H NMR spectroscopy experiments is presented. All the experimental parameters of NMR spectroscopy are also described. Details of the assignments of lipid components (qualitative analysis) and quantification of particular lipid components (quantitative analysis) are explained.

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Abbreviations

CHOL:

Free cholesterol

CHOLest:

Esterified cholesterol

DMD:

Duchenne Muscular Dystrophy

NMR:

Nuclear magnetic resonance

PL:

Phospholipids

TG:

Triglycerides

TSP:

[3-(trimethylsilyl) propionic-2, 2, 3, 3-d4 acid, sodium salt]

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Acknowledgment

The author wishes to thank University Grant Commission, Government of India, for their generous financial support [Grant number: No.F.4-2/2006 (BSR)/13-194/2008 (BSR)].

Author information

Authors and Affiliations

  1. School of Life Sciences (SLS), Jawaharlal Nehru University (JNU), JNU Ring Rd, New Delhi, 110067, USA

    Niraj Kumar Srivastava

Authors
  1. Niraj Kumar Srivastava
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Corresponding author

Correspondence to Niraj Kumar Srivastava .

Editor information

Editors and Affiliations

  1. Istituto di Anatomia Umana e Biologia Cellulare, Università Cattolica del Sacro Cuore, Roma, Italy

    Camilla Bernardini

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Srivastava, N.K. (2018). Proton Nuclear Magnetic Resonance (1H NMR) Spectroscopy-Based Analysis of Lipid Components in Serum/Plasma of Patients with Duchenne Muscular Dystrophy (DMD). In: Bernardini, C. (eds) Duchenne Muscular Dystrophy. Methods in Molecular Biology, vol 1687. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7374-3_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7374-3_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7373-6

  • Online ISBN: 978-1-4939-7374-3

  • eBook Packages: Springer Protocols

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