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Detecting Hematopoietic Stem Cell Proliferation Using BrdU Incorporation

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Cellular Quiescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1686))

Abstract

Cellular quiescence is a key component of hematopoietic stem cell (HSC) homeostasis; therefore, a reliable method to measure HSC cell division is critical in many studies. However, measuring the proliferation rate of largely quiescent and rare populations of cells can be challenging. Bromo-deoxyuridine (BrdU) incorporation into replicating DNA is a commonly used and highly reproducible method to detect cell division history. Here, we describe a protocol for BrdU incorporation analysis in hematopoietic stem and progenitor cells that can provide a sensitive measure of cell division even in rare cell populations. In combination with flow cytometry, this method can be generalized to analyze other cell populations and other tissues as identified by cell surface markers.

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Acknowledgments

The work summarized here was supported by grants from the NIDDK DK060445 (KAM) and the NHLBI K08HL098898, the Department of Defense IDEA award in bone marrow failure research (10505346), the Caroline Wiess Law Foundation for Molecular Medicine, the Aplastic Anemia and MDS International Foundation Liviya Anderson Award, and a March of Dimes Basil O’Connor Starter Scholar Award (KYK).

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Authors and Affiliations

  1. Pediatric Infectious Diseases, Stem Cells and Regenerative Medicine Center, Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX, USA

    Katie A. Matatall, Claudine S. Kadmon & Katherine Y. King

  2. Texas Children’s Hospital, 1102 Bates Street, Suite 1150, Houston, TX, 77030, USA

    Katherine Y. King

Authors
  1. Katie A. Matatall
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  2. Claudine S. Kadmon
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  3. Katherine Y. King
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Corresponding author

Correspondence to Katherine Y. King .

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Editors and Affiliations

  1. Texas Children’s Hospital, Baylor College of Medicine, Houston, Texas, USA

    H. Daniel Lacorazza

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Matatall, K.A., Kadmon, C.S., King, K.Y. (2018). Detecting Hematopoietic Stem Cell Proliferation Using BrdU Incorporation. In: Lacorazza, H. (eds) Cellular Quiescence. Methods in Molecular Biology, vol 1686. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7371-2_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7371-2_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7370-5

  • Online ISBN: 978-1-4939-7371-2

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