Abstract
The physiological characteristics of rat and murine hippocampal neurons are widely studied, especially because of the involvement of the hippocampus in learning, memory, and neurological functions. Primary cultures of hippocampal neurons are commonly used to discover cellular and molecular mechanisms in neurobiology. By isolating and culturing individual hippocampal neurons, neuroscientists are able to investigate the activity of neurons at the individual cell and single synapse level, and to analyze properties related to cellular structure, cellular trafficking, and individual protein subcellular localization or protein-protein interaction using a variety of biochemical techniques. Conclusions addressed from such research are critical for testing theories related to memory, learning, and neurological functions. Here, we will describe how to isolate and culture primary hippocampal cells from newborn mice. The hippocampus may be isolated from newborn mice in as short as 2 min, and the cell cultures can be maintained for up to 2 weeks, and then ready for investigation of subcellular localization of K+ channel proteins and interaction with SUMO-specific protease 2 (SENP2). The protocol provides a fast and efficient technique for the culture of neuronal cells from mouce hippocampal tissue, and will ensure the immunocytochemistry detection of subcellular localization or protein-protein interactions in neurological research.
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Acknowledgment
This work was supported by the National Natural Science Foundation of China (81671294 to Y.T.Q. and 81502531 to H.M.W.), the Natural Science Foundation of Shaanxi Province, China (2017JM3013 to Y.T.Q.), and the Fundamental Research Funds for the Central Universities (GK201603061 to Y.T.Q., GK201603064 to H.M.W., GK201603062 to H.J.S., GK201701005 to H.X., and 2016CSY014 to X.C.).
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Chen, X. et al. (2018). Hyper-SUMOylation of K+ Channels in Sudden Unexplained Death in Epilepsy: Isolation and Primary Culture of Dissociated Hippocampal Neurons from Newborn Mice for Subcellular Localization. In: Shyng, SL., Valiyaveetil, F., Whorton, M. (eds) Potassium Channels. Methods in Molecular Biology, vol 1684. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7362-0_6
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DOI: https://doi.org/10.1007/978-1-4939-7362-0_6
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7362-0
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