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Ion Binding to Transport Proteins using Isothermal Titration Calorimetry

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Potassium Channels

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1684))

Abstract

Isothermal titration calorimetry (ITC) is an emerging, label-free technology used to measure ligand binding to membrane proteins. This technology utilizes a titration calorimeter to measure the heat exchange upon ligands binding to proteins, the magnitude of which is based on the overall enthalpy of the reaction. In this protocol, the steps we and others use to measure ion binding to ion transport proteins are described.

The original version of this chapter was revised. An erratum to this chapter can be found at DOI 10.1007/978-1-4939-7362-0_25

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Acknowledgment

This work was supported by The Welch Foundation grant A-1742 and Texas A&M Startup Funds to S.W.L.

Author information

Authors and Affiliations

  1. Department of Biology, Texas A&M University, 3474 TAMU, College Station, TX, 77843-3474, USA

    Shian Liu & Steve W. Lockless

  2. Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA, 20148, USA

    Shian Liu

Authors
  1. Shian Liu
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  2. Steve W. Lockless
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Corresponding author

Correspondence to Steve W. Lockless .

Editor information

Editors and Affiliations

  1. Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, Oregon, USA

    Show-Ling Shyng

  2. Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, Oregon, USA

    Francis I. Valiyaveetil

  3. The Vollum Institute, Oregon Health and Science University, Portland, Oregon, USA

    Matt Whorton

1 Electronic Supplementary Material

Table S1

(XLSX 26 kb)

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Liu, S., Lockless, S.W. (2018). Ion Binding to Transport Proteins using Isothermal Titration Calorimetry. In: Shyng, SL., Valiyaveetil, F., Whorton, M. (eds) Potassium Channels. Methods in Molecular Biology, vol 1684. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7362-0_22

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  • DOI: https://doi.org/10.1007/978-1-4939-7362-0_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7361-3

  • Online ISBN: 978-1-4939-7362-0

  • eBook Packages: Springer Protocols

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