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Patch-Clamp Recordings of the KcsA K+ Channel in Unilamellar Blisters

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Potassium Channels

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1684))

Abstract

Patch-clamp electrophysiology is the standard technique used for the high-resolution functional measurements on ion channels. While studies using patch clamp are commonly carried out following ion channel expression in a heterologous system such as Xenopus oocytes or tissue culture cells, these studies can also be carried out using ion channels reconstituted into lipid vesicles. In this chapter, we describe the methodology for reconstituting ion channels into liposomes and the procedure for the generation of unilamellar blisters from these liposomes that are suitable for patch clamp. Here, we focus on the bacterial K+ channel KcsA, although the methodologies described in this chapter should be applicable for the functional analysis of other ion channels.

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Acknowledgment

This research was supported by a grant from the NIH (GM087546) to F.I.V.

Author information

Authors and Affiliations

  1. Program in Chemical Biology, Department of Physiology and Pharmacology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Mail Code L334, Portland, OR, 97239, USA

    Kimberly Matulef

  2. Department of Physiology and Pharmacology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Mail Code L334, Portland, OR, 97239, USA

    Francis I. Valiyaveetil

Authors
  1. Kimberly Matulef
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  2. Francis I. Valiyaveetil
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Corresponding author

Correspondence to Francis I. Valiyaveetil .

Editor information

Editors and Affiliations

  1. Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, Oregon, USA

    Show-Ling Shyng

  2. Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, Oregon, USA

    Francis I. Valiyaveetil

  3. The Vollum Institute, Oregon Health and Science University, Portland, Oregon, USA

    Matt Whorton

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Matulef, K., Valiyaveetil, F.I. (2018). Patch-Clamp Recordings of the KcsA K+ Channel in Unilamellar Blisters. In: Shyng, SL., Valiyaveetil, F., Whorton, M. (eds) Potassium Channels. Methods in Molecular Biology, vol 1684. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7362-0_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7362-0_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7361-3

  • Online ISBN: 978-1-4939-7362-0

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