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Translocation Biosensors—Versatile Tools to Probe Protein Functions in Living Cells

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High Content Screening

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1683))

Abstract

In this chapter, you will learn how to use translocation biosensors to investigate protein functions in living cells. We here present three classes of modular protein translocation biosensors tailored to investigate: (1) signal-mediated nucleo-cytoplasmic transport, (2) protease activity, and (3) protein-protein interactions. Besides the mapping of protein function, the biosensors are also applicable to identify chemicals and/or (nano) materials modulating the respective protein activities and can also be exploited for RNAi-mediated genetic screens.

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Author information

Authors and Affiliations

  1. Molecular and Cellular Oncology/ENT, University Medical Center, Langenbeckstr. 1, 55131, Mainz, Germany

    Verena Fetz & Roland H. Stauber

  2. Department of Chemical Biology, Helmholtz Institute of Infection Research, Braunschweig, Germany

    Verena Fetz

  3. Department of Experimental Toxicology and ZEBET, German Federal Institute for Risk Assessment (BfR), Diedersdorfer Weg 1, 12277, Berlin, Germany

    Verena Fetz

  4. Institute for Molecular Biology, Centre for Medical Biotechnology (ZMB), Mainz Screening Center UG & Co. KG, University of Duisburg-Essen, Essen, Germany

    Shirley K. Knauer

Authors
  1. Verena Fetz
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  2. Roland H. Stauber
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  3. Shirley K. Knauer
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Corresponding author

Correspondence to Verena Fetz .

Editor information

Editors and Affiliations

  1. Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

    Paul A. Johnston

  2. Bahama Bio, LLC, Bahama, North Carolina, USA

    Oscar J. Trask

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Fetz, V., Stauber, R.H., Knauer, S.K. (2018). Translocation Biosensors—Versatile Tools to Probe Protein Functions in Living Cells. In: Johnston, P., Trask, O. (eds) High Content Screening. Methods in Molecular Biology, vol 1683. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7357-6_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7357-6_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7355-2

  • Online ISBN: 978-1-4939-7357-6

  • eBook Packages: Springer Protocols

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