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Immunoelectron Microscopy for Visualization of Nanoparticles

  • Sarah R. Anderson
  • David Parmiter
  • Ulrich Baxa
  • Kunio NagashimaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1682)

Abstract

Immunoelectron microscopy (IEM) on a solid phase such as a carbon film is a fast and powerful way to detect and visualize surface antigens on nanoparticles by using a transmission electron microscope (TEM). Nanoparticles, in particular ones for medical applications, are often modified on the surface with soft materials to make them more soluble, less toxic, or targetable to cancerous tumors. Imaging the soft material on the surface of solid nanoparticles by electron microscopy is often a challenge. IEM can overcome this issue in cases where antibodies to any of the surface material are available, which is often the case for proteins, but also for commonly used materials such as polyethylene glycol (PEG). This effective procedure has been used traditionally for viruses and macromolecules, but it can be directly applied to nanoparticles.

Key words

Electron microscope Negative stain Immune electron microscopy Indirect solid phase immunolabeling Nanomaterial 

Notes

Acknowledgment

This project has been funded in whole or in part with Federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. HHSN261200800001E. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Sarah R. Anderson
    • 2
  • David Parmiter
    • 1
  • Ulrich Baxa
    • 1
  • Kunio Nagashima
    • 1
    Email author
  1. 1.Cancer Research Technology Program, Electron Microscopy LaboratoryLeidos Biomedical Research, Inc., Frederick National Laboratory for Cancer ResearchFrederickUSA
  2. 2.Microscopic Imaging Lab, Global Pathology, Drug Safety Research and DevelopmentPfizer, Inc.GrotonUSA

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