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Overview of Fluorescence Lifetime Measurements in Flow Cytometry

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Flow Cytometry Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1678))

Abstract

The focus of this chapter is time-resolved flow cytometry, which is broadly defined as the ability to measure the timing of fluorescence decay from excited fluorophores that pass through cytometers or high-throughput cell counting and cell sorting instruments. We focus on this subject for two main reasons: first, to discuss the nuances of hardware and software modifications needed for these measurements because currently, there are no widespread time-resolved cytometers nor a one-size-fits-all approach; and second, to summarize the application space for fluorescence lifetime-based cell counting/sorting owing to the recent increase in the number of investigators interested in this approach. Overall, this chapter is structured into three sections: (1) theory of fluorescence decay kinetics, (2) modern time-resolved flow cytometry systems, and (3) cell counting and sorting applications. These commentaries are followed by conclusions and discussion about new directions and opportunities for fluorescence lifetime measurements in flow cytometry.

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Authors and Affiliations

  1. Department of Chemical & Materials Engineering, New Mexico State University, MSC, PO Box 30001, Las Cruces, NM, 88003, USA

    Jessica P. Houston, Zhihua Yang, Jesse Sambrano, Wenyan Li & Kapil Nichani

  2. Kinetic River Corp., 897, Independence Avenue, Suite 4A, Mountain View, CA, 94043-2357, USA

    Giacomo Vacca

Authors
  1. Jessica P. Houston
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  2. Zhihua Yang
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  3. Jesse Sambrano
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  4. Wenyan Li
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  5. Kapil Nichani
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  6. Giacomo Vacca
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Corresponding author

Correspondence to Jessica P. Houston .

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Editors and Affiliations

  1. Flow Cytometry Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA

    Teresa S. Hawley

  2. Department of Anatomy and Regenerative Biology, School of Medicine and Health Sciences, George Washington University, Washington, District of Columbia, USA

    Robert G. Hawley

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Houston, J.P., Yang, Z., Sambrano, J., Li, W., Nichani, K., Vacca, G. (2018). Overview of Fluorescence Lifetime Measurements in Flow Cytometry. In: Hawley, T., Hawley, R. (eds) Flow Cytometry Protocols. Methods in Molecular Biology, vol 1678. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7346-0_18

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  • DOI: https://doi.org/10.1007/978-1-4939-7346-0_18

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