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Mapping of Histone Modifications in Plants by Tandem Mass Spectrometry

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Plant Chromatin Dynamics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1675))

Abstract

To get an insight into the mechanisms of gene expression regulation in eukaryotic organisms, it is necessary to decipher the connection between the different chemical modifications occurring on the chromatin, at both the DNA and the associated histone proteins. Histones are basic proteins, which pack the DNA into nucleosomes, and are hot spots for several posttranslational modifications. Elucidating combinatorial histone modifications co-occurring on the same histone protein will greatly contribute to our understanding of the mechanisms involved in the development of eukaryotes. The advancements in mass spectrometry technologies, including sensitivity, accuracy, and ionization strategies, have significantly contributed to the identification of novel single and combinatorial modifications on histones isolated from model organisms. In this chapter, we describe detailed protocols applied for the extraction, purification, and processing of histones for subsequent analysis by tandem mass spectrometry, using Brassica oleracea (cauliflower), a close relative of Arabidopsis thaliana.

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Authors and Affiliations

  1. Department of Plant Biology, Linnean Center for Plant Biology, Swedish University of Agricultural Sciences, Box 7080, 75007, Uppsala, Sweden

    Walid Mahrez & Lars Hennig

Authors
  1. Walid Mahrez
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  2. Lars Hennig
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Corresponding author

Correspondence to Lars Hennig .

Editor information

Editors and Affiliations

  1. Department of Molecular Biology, Wageningen University & Research, Wageningen, The Netherlands

    Marian Bemer

  2. Department of Plant and Microbial Biology, Zürich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland

    Célia Baroux

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Mahrez, W., Hennig, L. (2018). Mapping of Histone Modifications in Plants by Tandem Mass Spectrometry. In: Bemer, M., Baroux, C. (eds) Plant Chromatin Dynamics. Methods in Molecular Biology, vol 1675. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7318-7_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7318-7_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7317-0

  • Online ISBN: 978-1-4939-7318-7

  • eBook Packages: Springer Protocols

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