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Technical Review: Cytogenetic Tools for Studying Mitotic Chromosomes

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Plant Chromatin Dynamics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1675))

Abstract

Significant advances in chromosome preparation and other techniques have greatly increased the potential of plant cytogenetics in recent years. Increase in longitudinal resolution using DNA extended fibers as well as new developments in imaging and signal amplification technologies have enhanced the ability of FISH to detect small gene targets. The combination of fluorescence in situ hybridization with immunocytochemistry allows the investigation of cell events, chromosomal rearrangements and chromatin features typical for plant nuclei. Chromosome manipulation techniques using microdissection and flow sorting have accelerated the analysis of complex plant genomes. Together, the different cytogenetic approaches are invaluable for the unravelling of detailed structures of plant chromosomes, which are of utmost importance for the study of genome properties, DNA replication and gene regulation. In this technical review, different cytogenetic approaches are discussed for the analysis of plant chromosomes, with a focus on mitotic chromosomes.

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Acknowledgments

This research was supported by the Czech Science Foundation (grant 16-08698S). We greatly thank to Dr. Alexander Oulton for English revision of this chapter. We would like to thank to Dr. Jiri Siroky and Veronika Balounova for valuable comments.

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Authors and Affiliations

  1. Department of Plant Developmental Genetics, Institute of Biophysics, Czech Academy of Sciences, Brno, Czech Republic

    Václaclav Bačovský, Roman Hobza & Boris Vyskot

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  1. Václaclav Bačovský
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  2. Roman Hobza
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  3. Boris Vyskot
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Correspondence to Boris Vyskot .

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Editors and Affiliations

  1. Department of Molecular Biology, Wageningen University & Research, Wageningen, The Netherlands

    Marian Bemer

  2. Department of Plant and Microbial Biology, Zürich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland

    Célia Baroux

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Bačovský, V., Hobza, R., Vyskot, B. (2018). Technical Review: Cytogenetic Tools for Studying Mitotic Chromosomes. In: Bemer, M., Baroux, C. (eds) Plant Chromatin Dynamics. Methods in Molecular Biology, vol 1675. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7318-7_30

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  • DOI: https://doi.org/10.1007/978-1-4939-7318-7_30

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  • Print ISBN: 978-1-4939-7317-0

  • Online ISBN: 978-1-4939-7318-7

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