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Measuring Dynamics of Histone Proteins by Photobleaching in Arabidopsis Roots

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Plant Chromatin Dynamics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1675))

Abstract

Histone proteins play an important role in determining chromatin structure and gene expression. Studies in several systems have established that histones are in continuous turnover within the chromatin. It is therefore important to quantitatively measure the binding dynamics of these proteins in vivo. Photobleaching-based approaches such as fluorescence recovery after photobleaching (FRAP) are advantageous in that they are applied to living cells at a single cell level. In this chapter, I provide a detailed experimental protocol on how to perform histone FRAP experiments in Arabidopsis thaliana roots and how to analyze the most important parameters.

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Acknowledgments

This work was supported by the grant SFRH/BD/23202/2005 from the Portuguese Fundação para a Ciência e a Tecnologia (Portugal) and 3.3-GRO/1162118STP from the Humboldt Foundation (Germany). I thank Prof. Peter Shaw for helpful comments on the manuscript.

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Authors and Affiliations

  1. Institute of Biochemistry and Biology, Plant Physiology, University of Potsdam, Karl-Liebknecht-Str. 24-25, Building 20, DE-14476, Potsdam-Golm, Germany

    Stefanie Rosa

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  1. Stefanie Rosa
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Correspondence to Stefanie Rosa .

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Editors and Affiliations

  1. Department of Molecular Biology, Wageningen University & Research, Wageningen, The Netherlands

    Marian Bemer

  2. Department of Plant and Microbial Biology, Zürich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland

    Célia Baroux

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Rosa, S. (2018). Measuring Dynamics of Histone Proteins by Photobleaching in Arabidopsis Roots. In: Bemer, M., Baroux, C. (eds) Plant Chromatin Dynamics. Methods in Molecular Biology, vol 1675. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7318-7_26

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  • DOI: https://doi.org/10.1007/978-1-4939-7318-7_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7317-0

  • Online ISBN: 978-1-4939-7318-7

  • eBook Packages: Springer Protocols

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