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Plant Chromatin Dynamics pp 397–418Cite as

A Compendium of Methods to Analyze the Spatial Organization of Plant Chromatin

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1675))

Abstract

The long linear chromosomes of eukaryotic organisms are tightly packed into the nucleus of the cell. Beyond a first organization into nucleosomes and higher-order chromatin fibers, the positioning of nuclear DNA within the three-dimensional space of the nucleus plays a critical role in genome function and gene expression. Different techniques have been developed to assess nanoscale chromatin organization, nuclear position of genomic regions or specific chromatin features and binding proteins as well as higher-order chromatin organization. Here, I present an overview of imaging and molecular techniques applied to study nuclear architecture in plants, with special attention to the related protocols published in the “Plant Chromatin Dynamics” edition from Methods in Molecular Biology.

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Acknowledgments

I thank S. Desset and C. Tatout for critical reading and C. Baroux for helpful editorial suggestions. This work was supported by ANR grant ‘SINODYN’ ANR-12 ISV6 0001, the Centre National de la Recherche Scientifique, the Institut National de la Santé et de la Recherche Médicale and the University Clermont Auvergne.

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  1. GReD, Université Clermont Auvergne, CNRS, INSERM, 63001, Clermont-Ferrand, France

    Aline V. Probst

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  1. Aline V. Probst
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Correspondence to Aline V. Probst .

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  1. Department of Molecular Biology, Wageningen University & Research, Wageningen, The Netherlands

    Marian Bemer

  2. Department of Plant and Microbial Biology, Zürich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland

    Célia Baroux

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Probst, A.V. (2018). A Compendium of Methods to Analyze the Spatial Organization of Plant Chromatin. In: Bemer, M., Baroux, C. (eds) Plant Chromatin Dynamics. Methods in Molecular Biology, vol 1675. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7318-7_23

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  • DOI: https://doi.org/10.1007/978-1-4939-7318-7_23

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