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QTLepi Mapping in Arabidopsis thaliana

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Plant Chromatin Dynamics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1675))

Abstract

While DNA sequence variation is known to be a major driver of phenotypic divergence, epigenetic variation has long been disregarded. One reason for that was the lack of suitable tools. The creation of epigenetically divergent but otherwise largely isogenic Arabidopsis populations has now alleviated some of these constraints. Epigenetic recombinant inbred line (epiRIL) populations allow for examining the effects of epigenetic variation on phenotypes. In addition, epiRILs enabled the development of epigenetic quantitative trait locus (QTLepi) mapping, an approach to identify causal epigenetic factors. Here, we describe the successive steps of QTLepi mapping in a broad sense, from the creation of epigenetically divergent populations to the identification of causal genes underlying particular phenotypes in Arabidopsis.

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Acknowledgment

K.L. was supported by the Centre for Improving Plant Yield (CIPY), which is part of the Netherlands Genomics Initiative and the Netherlands Organization for Scientific Research.

Author information

Authors and Affiliations

  1. Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098XH, Amsterdam, The Netherlands

    Kathrin Lauss

  2. Laboratory of Genetics, Wageningen University and Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands

    Joost J. B. Keurentjes

Authors
  1. Kathrin Lauss
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  2. Joost J. B. Keurentjes
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Corresponding author

Correspondence to Joost J. B. Keurentjes .

Editor information

Editors and Affiliations

  1. Department of Molecular Biology, Wageningen University & Research, Wageningen, The Netherlands

    Marian Bemer

  2. Department of Plant and Microbial Biology, Zürich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland

    Célia Baroux

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Lauss, K., Keurentjes, J.J.B. (2018). QTLepi Mapping in Arabidopsis thaliana . In: Bemer, M., Baroux, C. (eds) Plant Chromatin Dynamics. Methods in Molecular Biology, vol 1675. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7318-7_22

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  • DOI: https://doi.org/10.1007/978-1-4939-7318-7_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7317-0

  • Online ISBN: 978-1-4939-7318-7

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