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Genome Instability pp 101–105Cite as

Study of UV-induced DNA Repair Factor Recruitment: Kinetics and Dynamics

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1672))

Abstract

The local UV irradiation technique enables detection, kinetic measurements of recruitment, and quantification of DNA Damage Response (DDR) proteins at the site of UV-induced DNA damage.

Using Isopore filters with high density pores of a broad range of sizes, it is possible to UV irradiate and damage only a very small portion of the nucleus of a cell by letting UV light pass only through the pores. Immunofluorescent analyses of modified DNA nucleotides, proteins, or fluorescently tagged versions of target factors can be used as markers to label and study UV-induced lesions and their repair.

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Authors and Affiliations

  1. Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, Milano, 20133, Italy

    Sarah Sertic, Stefania Roma, Paolo Plevani, Federico Lazzaro & Marco Muzi-Falconi

Authors
  1. Sarah Sertic
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  2. Stefania Roma
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  3. Paolo Plevani
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  4. Federico Lazzaro
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  5. Marco Muzi-Falconi
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Corresponding author

Correspondence to Marco Muzi-Falconi .

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Editors and Affiliations

  1. Dipartimento di Bioscienze, Università degli Studi di Milano, Milano, Milano, Italy

    Marco Muzi-Falconi

  2. Donnelly Centre and Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada

    Grant W Brown

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Sertic, S., Roma, S., Plevani, P., Lazzaro, F., Muzi-Falconi, M. (2018). Study of UV-induced DNA Repair Factor Recruitment: Kinetics and Dynamics. In: Muzi-Falconi, M., Brown, G. (eds) Genome Instability. Methods in Molecular Biology, vol 1672. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7306-4_8

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  • DOI: https://doi.org/10.1007/978-1-4939-7306-4_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7305-7

  • Online ISBN: 978-1-4939-7306-4

  • eBook Packages: Springer Protocols

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