Abstract
Ultrafine anaphase bridges (UFBs) are thin DNA threads linking the separating sister chromatids in the anaphase of mitosis. UFBs are thought to form when topological DNA entanglements between two chromatids are not resolved prior to anaphase onset. In contrast to other markers of defective chromosome segregation, UFBs cannot be detected by direct staining of the DNA, but instead can be detected using immunofluorescence-based approaches. Due to the fact that they are short-lived and fragile in nature, UFBs can be challenging to detect. In this chapter, we describe methods that have been optimized for successful detection of UFBs. We also provide guidelines for the optimization of UFBs detection depending on the antibody and the cell line to be used.
The original version of this chapter was revised. An erratum to this chapter can be found at DOI 10.1007/978-1-4939-7306-4_43
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Acknowledgments
Work in the authors’ laboratory is supported by The European Research Council and The Danish National Research Foundation (grant DNRF115).
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Bizard, A.H., Nielsen, C.F., Hickson, I.D. (2018). Detection of Ultrafine Anaphase Bridges. In: Muzi-Falconi, M., Brown, G. (eds) Genome Instability. Methods in Molecular Biology, vol 1672. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7306-4_33
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DOI: https://doi.org/10.1007/978-1-4939-7306-4_33
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