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A qPCR-Based Protocol to Quantify DSB Resection

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Genome Instability

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1672))

Abstract

The nucleolytic degradation of the 5′-ending strand of a Double-Strand DNA break (DSB) is necessary to initiate homologous recombination to correctly repair the break. This process is called DNA end resection and it is finely regulated to prevent genome rearrangements. Here, we describe a protocol to quantify DSB resection rate by qPCR, which could be applied to every organisms whenever the break site and its flanking region sequences are known.

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References

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Acknowledgments

This work was supported by grants from Associazione Italiana per la Ricerca sul Cancro [AIRC_IG Grant n.15488 to A.P.; CARIPLO [2013-0790 to A.P.]; a fellowship from Fondazione “Gabriella Dolfin Voyasidis”-Accademia Nazionale dei Lincei to M.F.

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Authors and Affiliations

  1. Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133, Milano, Italy

    Matteo Ferrari, Shyam Twayana, Federica Marini & Achille Pellicioli

Authors
  1. Matteo Ferrari
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  2. Shyam Twayana
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  3. Federica Marini
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  4. Achille Pellicioli
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Corresponding author

Correspondence to Achille Pellicioli .

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Editors and Affiliations

  1. Dipartimento di Bioscienze, Università degli Studi di Milano, Milano, Milano, Italy

    Marco Muzi-Falconi

  2. Donnelly Centre and Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada

    Grant W Brown

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Ferrari, M., Twayana, S., Marini, F., Pellicioli, A. (2018). A qPCR-Based Protocol to Quantify DSB Resection. In: Muzi-Falconi, M., Brown, G. (eds) Genome Instability. Methods in Molecular Biology, vol 1672. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7306-4_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7306-4_10

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7305-7

  • Online ISBN: 978-1-4939-7306-4

  • eBook Packages: Springer Protocols

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