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The A-Like Faker Assay for Measuring Yeast Chromosome III Stability

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Genome Instability

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1672))

Abstract

The ability to rapidly assess chromosome instability (CIN) has enabled profiling of most yeast genes for potential effects on genome stability. The A-like faker (ALF) assay is one of several qualitative and quantitative marker loss assays that indirectly measure loss or conversion of genetic material using a counterselection step. The ALF assay relies on the ability to count spurious mating events that occur upon loss of the MATĪ± locus of haploid Saccharomyces cerevisiae strains. Here, we describe the deployment of the ALF assay for both rapid and simple qualitative, and more in-depth quantitative analysis allowing determination of absolute ALF frequencies.

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Acknowledgments

We thank Philip Hieter for helpful discussions. PCS is a Canadian Institutes of Health Research (CIHR) New Investigator and a Michael Smith Foundation for Health Research Scholar. We acknowledge operating support from CIHR (MOP-136982) and this research is funded by the Canadian Cancer Society (grant 703263).

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Authors and Affiliations

  1. Terry Fox Laboratory, BC Cancer Agency, 675 W. 10th Ave, Vancouver, Canada

    Carolina A. NovoaĀ &Ā Peter C. Stirling

  2. Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, Canada

    J. Sidney Ang

  3. Department of Medical Genetics, University of British Columbia, Vancouver, Canada

    Peter C. Stirling

Authors
  1. Carolina A. Novoa
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  2. J. Sidney Ang
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  3. Peter C. Stirling
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Corresponding author

Correspondence to Peter C. Stirling .

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Editors and Affiliations

  1. Dipartimento di Bioscienze, UniversitĆ  degli Studi di Milano, Milano, Milano, Italy

    Marco Muzi-Falconi

  2. Donnelly Centre and Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada

    Grant W Brown

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Novoa, C.A., Ang, J.S., Stirling, P.C. (2018). The A-Like Faker Assay for Measuring Yeast Chromosome III Stability. In: Muzi-Falconi, M., Brown, G. (eds) Genome Instability. Methods in Molecular Biology, vol 1672. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7306-4_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7306-4_1

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7305-7

  • Online ISBN: 978-1-4939-7306-4

  • eBook Packages: Springer Protocols

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