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Designing and Implementing Algorithmic DNA Assembly Pipelines for Multi-Gene Systems

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Synthetic Metabolic Pathways

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1671))

Abstract

Advances in DNA synthesis and assembly technology allow for the high-throughput fabrication of hundreds to thousands of multi-part genetic constructs in a short time. This allows for rapid hypothesis-testing and genetic optimization in multi-gene biological systems. Here, we discuss key considerations to design and implement an algorithmic DNA assembly pipeline that provides the freedom to change nearly any design variable in a multi-gene system. In addition to considerations for pipeline design, we describe protocols for three useful molecular biology techniques in plasmid construction.

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Acknowledgment

S-Y. H. is supported by Biocatalysis Grant from the University of Minnesota BioTechnology Institute.

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Authors and Affiliations

  1. Department of Biochemistry, Molecular Biology, and Biophysics and BioTechnology Institute, University of Minnesota, Twin Cities, Saint Paul, MN, 55108, USA

    Szu-Yi Hsu & Michael J. Smanski

Authors
  1. Szu-Yi Hsu
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  2. Michael J. Smanski
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Corresponding author

Correspondence to Michael J. Smanski .

Editor information

Editors and Affiliations

  1. The Novo Nordisk Foundation, Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark

    Michael Krogh Jensen

  2. Joint BioEnergy Institute, Emeryville, California, USA

    Jay D. Keasling

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Hsu, SY., Smanski, M.J. (2018). Designing and Implementing Algorithmic DNA Assembly Pipelines for Multi-Gene Systems. In: Jensen, M.K., Keasling, J.D. (eds) Synthetic Metabolic Pathways. Methods in Molecular Biology, vol 1671. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7295-1_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7295-1_9

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7294-4

  • Online ISBN: 978-1-4939-7295-1

  • eBook Packages: Springer Protocols

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