Abstract
Chickpea is an important leguminous crop that belongs to Fabaceae family, highly valued for its nutritious seeds. Seeds contain reserve food for the developing embryos. Mitochondria are crucial organelle for generation of chemical energy in the form of ATP which is required for achieving metabolically active state; therefore, investigating mitochondrial function and respiration rate is crucial for exploring various metabolic and physio-biochemical changes that occur during seed germination. Here we describe a method for isolation of mitochondria from germinating seeds of two chickpea varieties, i.e., Desi and Kabuli. Structure of Mitotracker-stained isolated mitochondria was observed by confocal microscopy and respiration rate was measured using an oxygen microsensor.
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References
FAO (2003) http://www.fao.org. Production database
Carranca C, De Varennes A, Rolston D (1999) Biological nitrogen fixation by fababean, pea and chickpea, under field conditions, estimated by the 15N isotope dilution technique. Eur J Agron 10:49–56
Jukanti AK, Gaur PM, Gowda CLL, Chibbar RN (2012) Nutritional quality and health benefits of chickpea (Cicer arietinum L.): a review. Br J Nutr 108:S11–S26
Pradhan S, Bandhiwal N, Shah N, Kant C, Gaur R, Bhatia S (2014) Global transcriptome analysis of developing chickpea (Cicer arietinum L.) seeds. Front Plant Sci 5:698
van der Maesen LJG (1972) A monograph of the genus, with special references to chickpea (Cicer arietinum L.). Its ecology and cultivation. Mededlingen Landbouw Hogeschool (Communication Agricultural University), Wageningen
Martín JJ, Tocino Á, Ardanuy R, Juana G, Cervantes E (2014) Dynamic analysis of Arabidopsis seed shape reveals differences in cellulose mutants. Acta Physiol Plant 36:1585–1592
Bowsher CG, Tobin AK (2001) Compartmentation of metabolism within mitochondria and plastids. J Exp Bot 52:513–527
Logan DC, Millar AH, Sweetlove LJ, Hill SA, Leaver CJ (2001) Mitochondrial biogenesis during germination in maize embryos. Plant Physiol 125:662–672
Acknowledgements
This work was supported by NIPGR short term research fellowship to S.P., and IYBA and Ramalingaswami to K.J.G. from Department of Biotechnology.
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Pandey, S., Kumari, A., Gupta, K.J. (2017). Isolation of Physiologically Active and Intact Mitochondria from Chickpea. In: Jagadis Gupta, K. (eds) Plant Respiration and Internal Oxygen. Methods in Molecular Biology, vol 1670. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7292-0_9
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DOI: https://doi.org/10.1007/978-1-4939-7292-0_9
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