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Using an Oxygen Microsensor to Measure Oxygen Dynamics in Tomato Plants in Response to Pseudomonas syringae Infection

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Plant Respiration and Internal Oxygen

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1670))

Abstract

Pathogen infection leads to induction of defense responses which includes modulation of gene expression and changes in metabolism plants. Despite of extensive research little is information known about the role of respiration and photosynthesis during pathogen infection in plants. Limited methods are available to measure oxygen dynamics in response to pathogen infection. Here by using an oxygen microsensor we measured oxygen changes in tomato plants infected with avirulent Pseudomonas syringae pv. tomato DC3000. In this method plant is placed in a closed chamber and change in oxygen levels can be measured by an oxygen sensor.

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Acknowledgement

KJG is grateful to Ramalingaswami reentry fellowship and IYBA from DBT. PKP is the recipient of UGC-JRF.

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Authors and Affiliations

  1. National Institute of Plant Genome Research, Aruna Asaf Ali Marg, PO Box 10531, 10067, New Delhi, India

    Pradeep Kumar Pathak & Kapuganti Jagadis Gupta

Authors
  1. Pradeep Kumar Pathak
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  2. Kapuganti Jagadis Gupta
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Corresponding author

Correspondence to Kapuganti Jagadis Gupta .

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Editors and Affiliations

  1. National Institute of Plant Genome Research, New Delhi, Delhi, India

    Kapuganti Jagadis Gupta

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Pathak, P.K., Gupta, K.J. (2017). Using an Oxygen Microsensor to Measure Oxygen Dynamics in Tomato Plants in Response to Pseudomonas syringae Infection. In: Jagadis Gupta, K. (eds) Plant Respiration and Internal Oxygen. Methods in Molecular Biology, vol 1670. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7292-0_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7292-0_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7291-3

  • Online ISBN: 978-1-4939-7292-0

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