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Plant Respiration and Internal Oxygen pp 167–182Cite as

Measurement of Tricarboxylic Acid Cycle Enzyme Activities in Plants

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1670))

Abstract

Mitochondria are vital cytoplasmic organelle of eukaryotic cells responsible for oxidative energy metabolism and the synthesis of intermediates utilized in various other metabolic pathways. The functions of mitochondrion are the oxidation of organic acids by the tricarboxylic acid (TCA) cycle and the synthesis of ATP by the oxidative phosphorylation in the mitochondrial electron transport chain. The TCA cycle is composed by a set of enzymes that are essential for optimal functioning of the primary carbon metabolism in plants. The activity of each TCA cycle enzyme in plants may vary according to cell type, plant tissue, stage of plant development, and the environment. Here, we describe current methods used for the determination of the TCA cycle enzyme activities in different plant tissues.

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Acknowledgements

Financial support was provided by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq- to W.L.A.), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), and Max Planck Society to A.N.N. and W.L.A. Research fellowships granted by CNPq to A.N.N. and W.L.A. as well as scholarship granted by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) to R.P.O.G. are also gratefully acknowledged.

Conflict of interest : The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-900, Viçosa, Brazil

    Rebeca Patricia Omena-Garcia, Wagner L. Araújo & Adriano Nunes-Nesi

  2. UMR 1332 Biologie du Fruit et Pathologie, INRA, Villenave d’ Ornon, F-33883, France

    Yves Gibon

  3. Max Planck Institute of Molecular Plant Physiology, Wissenschaftspark Golm, 14476, Potsdam-Golm, Germany

    Alisdair R. Fernie

Authors
  1. Rebeca Patricia Omena-Garcia
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  2. Wagner L. Araújo
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  3. Yves Gibon
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  4. Alisdair R. Fernie
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  5. Adriano Nunes-Nesi
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Corresponding author

Correspondence to Alisdair R. Fernie .

Editor information

Editors and Affiliations

  1. National Institute of Plant Genome Research, New Delhi, Delhi, India

    Kapuganti Jagadis Gupta

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Omena-Garcia, R.P., Araújo, W.L., Gibon, Y., Fernie, A.R., Nunes-Nesi, A. (2017). Measurement of Tricarboxylic Acid Cycle Enzyme Activities in Plants. In: Jagadis Gupta, K. (eds) Plant Respiration and Internal Oxygen. Methods in Molecular Biology, vol 1670. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7292-0_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7292-0_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7291-3

  • Online ISBN: 978-1-4939-7292-0

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