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Isolation and Structural Studies of Mitochondria from Pea Roots

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Plant Respiration and Internal Oxygen

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1670))

Abstract

For structural and respiratory studies, isolation of intact and active mitochondria is essential. Here, we describe an isolation method which gave good yield and intact mitochondria from 2-week-old pea (Pisum sativum) roots grown hydroponically under standard growth conditions. We used Percoll gradient centrifugation for this isolation procedure. The yield of purified mitochondria was 50 μg/g FW. Isolated mitochondria maintained their structure which was observed by using MitoTracker green in confocal microscope and scanning electron microscopy (SEM). Intact mitochondria are clearly visible in SCM images. Taken together this isolation method can be used for physiological and microscopic studies on mitochondria.

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Acknowledgements

A.P.V. is currently funded by NPDF program of SERB, DST, India. We thank Aakanksha Wany for help in confocal microscopy.

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Authors and Affiliations

  1. National Institute of Plant Genome Research, Aruna Asaf Ali Marg, PO Box 10531, New Delhi, 110067, India

    Abhaypratap Vishwakarma & Kapuganti Jagadis Gupta

Authors
  1. Abhaypratap Vishwakarma
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  2. Kapuganti Jagadis Gupta
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Correspondence to Kapuganti Jagadis Gupta .

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Editors and Affiliations

  1. National Institute of Plant Genome Research, New Delhi, Delhi, India

    Kapuganti Jagadis Gupta

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Vishwakarma, A., Gupta, K.J. (2017). Isolation and Structural Studies of Mitochondria from Pea Roots. In: Jagadis Gupta, K. (eds) Plant Respiration and Internal Oxygen. Methods in Molecular Biology, vol 1670. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7292-0_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7292-0_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7291-3

  • Online ISBN: 978-1-4939-7292-0

  • eBook Packages: Springer Protocols

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