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Plant Germline Development pp 387–406Cite as

In Vivo Reporters for Protein Half-Life

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1669))

Abstract

Determination of the general capacity of proteolytic activity of a certain cell or tissue type can be crucial for an assessment of various features of an organism’s growth and development and also for the optimization of biotechnological applications. Here, we describe the use of chimeric protein stability reporters that can be detected by standard laboratory techniques such as histological staining, selection using selective media or fluorescence microscopy. Dependent on the expression of the reporters due to the promoters applied, cell- and tissue-specific questions can be addressed. Here, we concentrate on methods which can be used for large-scale screening for protein stability changes rather than for detailed protein stability studies.

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Acknowledgements

This work was supported by a grant for setting up the junior research group of the ScienceCampus Halle – Plant-based Bioeconomy to N.D., a Ph.D. scholarship from the DAAD (Deutscher Akademischer Austauschdienst) to P.R., by DI 1794/3-1 of the German Research Foundation (DFG) to N.D., and by grant LSP-TP2-1 of the Research Focus Program “Molecular Biosciences as a Motor for a Knowledge-Based Economy” from the European Regional Development Fund (EFRE) to N.D. Financial support came from the Leibniz Association, the state of Saxony-Anhalt, the DFG Graduate Training Center GRK1026 “Conformational Transitions in Macromolecular Interactions” at Halle, and the Leibniz Institute of Plant Biochemistry (IPB) at Halle, Germany. N.D. lab is the participant of the European Cooperation in Science and Technology (COST) Action BM1307—“European network to integrate research on intracellular proteolysis pathways in health and disease (PROTEOSTASIS)”.

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  1. Independent Junior Research Group on Protein Recognition and Degradation, Leibniz Institute of Plant Biochemistry (IPB) and Science Campus Halle – Plant-Based Bioeconomy, Halle (Saale), Germany

    Pavel Reichman & Nico Dissmeyer

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  1. Pavel Reichman
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  2. Nico Dissmeyer
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Correspondence to Nico Dissmeyer .

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  1. Centre for Organismal Studies, University of Heidelberg, Heidelberg, Germany

    Anja Schmidt

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Reichman, P., Dissmeyer, N. (2017). In Vivo Reporters for Protein Half-Life. In: Schmidt, A. (eds) Plant Germline Development. Methods in Molecular Biology, vol 1669. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7286-9_29

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  • DOI: https://doi.org/10.1007/978-1-4939-7286-9_29

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7285-2

  • Online ISBN: 978-1-4939-7286-9

  • eBook Packages: Springer Protocols

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