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Transgenic Reproductive Cell Ablation

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Plant Germline Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1669))

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Abstract

Numerous cell ablation technologies are available and have been used in reproductive tissues, particularly for male tissues and cells. The importance of ablation of reproductive tissues is toward a fundamental understanding reproductive tissue development and fertilization, as well as, in developing sterility lines important to breeding strategies. Here, we describe techniques for developing ablation lines for both male and female reproductive cells. Also discussed are techniques for analysis, quality control, maintenance, and the lessening of pleiotropism in such lines.

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Acknowledgements

This work was developed at and supported by DuPont Pioneer. We thank Katherine Thilges, Eric Caswell, and others for technical support of this work.

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Authors and Affiliations

  1. DuPont Pioneer, 7250 NW 62nd Ave., Johnston, IA, 50131-0552, USA

    Shai J. Lawit & Mark A. Chamberlin

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  1. Shai J. Lawit
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  2. Mark A. Chamberlin
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Correspondence to Shai J. Lawit .

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Editors and Affiliations

  1. Centre for Organismal Studies, University of Heidelberg, Heidelberg, Germany

    Anja Schmidt

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Lawit, S.J., Chamberlin, M.A. (2017). Transgenic Reproductive Cell Ablation. In: Schmidt, A. (eds) Plant Germline Development. Methods in Molecular Biology, vol 1669. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7286-9_28

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  • DOI: https://doi.org/10.1007/978-1-4939-7286-9_28

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7285-2

  • Online ISBN: 978-1-4939-7286-9

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