Abstract
Apomixis, commonly defined as asexual reproduction through seed, is a reproductive trait that occurs in only a few minor crops, but would be highly valuable in major crops. Apomixis results in seed-derived progenies that are genetically identical to their maternal parent. The advantage of apomixis would lie in seed propagation of elite food, feed, and biofuel crops that are heterozygous such as hybrid corn and switchgrass or self-pollinating crops for which no commercial-scale hybrid production system is available. While hybrid plants often outperform parental lines in growth and higher yields, production of hybrid seed is accomplished through carefully controlled, labor intensive crosses. Both small farmers in developing countries who produce their own seed and commercial companies that market hybrid seed could benefit from the establishment of engineered apomixis in plants. In this chapter, we review what has been learned from studying natural apomicts and mutations in sexual plants leading to apomixis-like development, plus discuss how the components of apomixis could be successfully engineered in plants.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Asker S, Jerling L (1992) Apomixis in plants. CRC Press, Boca Raton, FL
Hojsgaard D, Klatt S, Baier R, Carman JG, Hörandl E (2014) Taxonomy and biogeography of apomixis in angiosperms and associated biodiversity characteristics. Crit Rev Plant Sci 33(5):414–427
Noyes RD, Wagner JD (2014) Dihaploidy yields diploid apomicts and parthenogens in Erigeron (Asteraceae). Am J Bot 101(5):865–874
Voigt-Zielinski M-L, Piwczyński M, Sharbel TF (2012) Differential effects of polyploidy and diploidy on fitness of apomictic Boechera. Sex Plant Reprod 25(2):97–109
Grimanelli D, Leblanc O, Espinosa E, Perotti E, Gonzalez De Leon D, Savidan Y (1998) Mapping diplosporous apomixis in tetraploid Tripsacum: one gene or several genes? Heredity 80(1):33–39
Leblanc O, Grimanelli D, Gonzalez-de-Leon D, Savidan Y (1995) Detection of the apomictic mode of reproduction in maize-Tripsacum hybrids using maize RFLP markers. Theor Appl Genet 90(7–8):1198–1203
Pessino S, Ortiz J, Leblanc O, Do Valle C, Evans C, Hayward M (1997) Identification of a maize linkage group related to apomixis in Brachiaria. Theor Appl Genet 94(3–4):439–444
Pessino SC, Evans C, Ortiz JPA, Armstead I, Valle CBD, Hayward MD (1998) A genetic map of the apospory-region in Brachiaria hybrids: identification of two markers closely associated with the trait. Hereditas 128(2):153–158
Zorzatto C, Chiari L, De Araújo Bitencourt G, Do Valle C, De Campos Leguizamón G, Schuster I, Pagliarini M (2010) Identification of a molecular marker linked to apomixis in Brachiaria humidicola (Poaceae). Plant Breed 129(6):734–736
Thaikua S, Ebina M, Yamanaka N, Shimoda K, Suenaga K, Kawamoto Y (2016) Tightly clustered markers linked to an apospory-related gene region and quantitative trait loci mapping for agronomic traits in Brachiaria hybrids. Grassl Sci 62(2):69–80
Worthington M, Heffelfinger C, Bernal D, Quintero C, Zapata YP, Perez JG, De Vega J, Miles J, Dellaporta S, Tohme J (2016) A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic Brachiaria decumbens. Genetics 116:190314
Gustine D, Sherwood R, Huff D (1997) Apospory-linked molecular markers in buffelgrass. Crop Sci 37(3):947–951
Ozias-Akins P, Roche D, Hanna WW (1998) Tight clustering and hemizygosity of apomixis-linked molecular markers in Pennisetum squamulatum implies genetic control of apospory by a divergent locus that may have no allelic form in sexual genotypes. Proc Natl Acad Sci 95(9):5127–5132
Roche D, Cong P, Chen Z, Hanna WW, Gustine DL, Sherwood RT, Ozias-Akins P (1999) An apospory-specific genomic region is conserved between Buffelgrass (Cenchrus ciliaris L.) and Pennisetum squamulatum Fresen. Plant J 19(2):203–208
Jessup R, Burson B, Burow G, Wang Y-W, Chang C, Li Z, Paterson A, Hussey M (2002) Disomic inheritance, suppressed recombination, and allelic interactions govern apospory in buffelgrass as revealed by genome mapping. Crop Sci 42(5):1688–1694
Goel S, Chen Z, Conner JA, Akiyama Y, Hanna WW, Ozias-Akins P (2003) Delineation by fluorescence in situ hybridization of a single hemizygous chromosomal region associated with aposporous embryo sac formation in Pennisetum squamulatum and Cenchrus ciliaris. Genetics 163(3):1069–1082
Pupilli F, Labombarda P, Caceres ME, Quarín CL, Arcioni S (2001) The chromosome segment related to apomixis in Paspalum simplex is homoeologous to the telomeric region of the long arm of rice chromosome 12. Mol Breed 8(1):53–61
Labombarda P, Busti A, Caceres ME, Pupilli F, Arcioni S (2002) An AFLP marker tightly linked to apomixis reveals hemizygosity in a portion of the apomixis-controlling locus in Paspalum simplex. Genome 45(3):513–519
Martínez EJ, Hopp HE, Stein J, Ortiz JP, Quarin CL (2003) Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers. Mol Breed 12(4):319–327
Stein J, Pessino SC, Martínez EJ, Rodriguez MP, Siena LA, Quarin CL, Ortiz JPA (2007) A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers. Mol Breed 20(2):153–166
Ebina M, Nakagawa H, Yamamoto T, Araya H, Si T, Takahara M, Nakajima K (2005) Co-segregation of AFLP and RAPD markers to apospory in Guineagrass (Panicum maximum Jacq.) Grassl Sci 51(1):71–78
Barcaccia G, Mazzucato A, Albertini E, Zethof J, Gerats A, Pezzotti M, Falcinelli M (1998) Inheritance of parthenogenesis in Poa pratensis L.: auxin test and AFLP linkage analyses support monogenic control. Theor Appl Genet 97(1–2):74–82
Porceddu A, Albertini E, Barcaccia G, Falistocco E, Falcinelli M (2002) Linkage mapping in apomictic and sexual Kentucky bluegrass (Poa pratensis L.) genotypes using a two way pseudo-testcross strategy based on AFLP and SAMPL markers. Theor Appl Genet 104(2–3):273–280
Albertini E, Barcaccia G, Porceddu A, Sorbolini S, Falcinelli M (2001) Mode of reproduction is detected by Parth1 and Sex1 SCAR markers in a wide range of facultative apomictic Kentucky bluegrass varieties. Mol Breed 7(4):293–300
Conner JA, Gunawan G, Ozias-Akins P (2013) Recombination within the apospory specific genomic region leads to the uncoupling of apomixis components in Cenchrus ciliaris. Planta 238(1):51–63
Kaushal P, Malaviya D, Roy A, Pathak S, Agrawal A, Khare A, Siddiqui S (2008) Reproductive pathways of seed development in apomictic guinea grass (Panicum maximum Jacq.) reveal uncoupling of apomixis components. Euphytica 164(1):81–92
Catanach AS, Erasmuson SK, Podivinsky E, Jordan BR, Bicknell R (2006) Deletion mapping of genetic regions associated with apomixis in Hieracium. Proc Natl Acad Sci 103(49):18650–18655
Koltunow AM, Johnson SD, Rodrigues J, Okada T, Hu Y, Tsuchiya T, Wilson S, Fletcher P, Ito K, Suzuki G (2011) Sexual reproduction is the default mode in apomictic Hieracium subgenus Pilosella, in which two dominant loci function to enable apomixis. Plant J 66(5):890–902
van Dijk PJ, Bakx-Schotman JT (2004) Formation of unreduced megaspores (diplospory) in apomictic dandelions (Taraxacum officinale, sl) is controlled by a sex-specific dominant locus. Genetics 166(1):483–492
Noyes RD, Rieseberg LH (2000) Two independent loci control agamospermy (apomixis) in the triploid flowering plant Erigeron annuus. Genetics 155(1):379–390
Noyes R, Baker R, Mai B (2007) Mendelian segregation for two-factor apomixis in Erigeron annuus (Asteraceae). Heredity 98(2):92–98
Schallau A, Arzenton F, Johnston AJ, Hähnel U, Koszegi D, Blattner FR, Altschmied L, Haberer G, Barcaccia G, Bäumlein H (2010) Identification and genetic analysis of the APOSPORY locus in Hypericum perforatum L. Plant J 62(5):773–784
Ogawa D, Johnson SD, Henderson ST, Koltunow AM (2013) Genetic separation of autonomous endosperm formation (AutE) from the two other components of apomixis in Hieracium. Plant Reprod 26(2):113–123
van Dijk PJ, van Baarlen P, De Jong JH (2003) The occurrence of phenotypically complementary apomixis-recombinants in crosses between sexual and apomictic dandelions (Taraxacum officinale). Sex Plant Reprod 16(2):71–76
Nakano M, Shimada T, Endo T, Fujii H, Nesumi H, Kita M, Ebina M, Shimizu T, Omura M (2012) Characterization of genomic sequence showing strong association with polyembryony among diverse citrus species and cultivars, and its synteny with Vitis and Populus. Plant Sci 183:131–142
Kepiro J, Roose M (2010) AFLP markers closely linked to a major gene essential for nucellar embryony (apomixis) in Citrus maxima× Poncirus trifoliata. Tree Genet Genomes 6(1):1–11
Silveira ÉD, Guimarães LA, de Alencar Dusi DM, Da Silva FR, Martins NF, do Carmo Costa MM, Alves-Ferreira M, de Campos Carneiro VT (2012) Expressed sequence-tag analysis of ovaries of Brachiaria brizantha reveals genes associated with the early steps of embryo sac differentiation of apomictic plants. Plant Cell Rep 31(2):403–416
Rodrigues JC, Cabral GB, Dusi DM, de Mello LV, Rigden DJ, Carneiro VT (2003) Identification of differentially expressed cDNA sequences in ovaries of sexual and apomictic plants of Brachiaria brizantha. Plant Mol Biol 53(6):745–757
Vielle-Calzada J-P, Nuccio ML, Budiman MA, Thomas TL, Burson BL, Hussey MA, Wing RA (1996) Comparative gene expression in sexual and apomictic ovaries of Pennisetum ciliare (L.) Link. Plant Mol Biol 32(6):1085–1092
Singh M, Burson BL, Finlayson SA (2007) Isolation of candidate genes for apomictic development in buffelgrass (Pennisetum ciliare). Plant Mol Biol 64(6):673–682
Pessino SC, Espinoza F, Martinez EJ, Ortiz JPA, Valle EM, Quarin CL (2001) Isolation of cDNA clones differentially expressed in flowers of apomictic and sexual Paspalum notatum. Hereditas 134(1):35–42
Laspina NV, Vega T, Seijo JG, González AM, Martelotto LG, Stein J, Podio M, Ortiz JPA, Echenique VC, Quarin CL (2008) Gene expression analysis at the onset of aposporous apomixis in Paspalum notatum. Plant Mol Biol 67(6):615–628
Polegri L, Calderini O, Arcioni S, Pupilli F (2010) Specific expression of apomixis-linked alleles revealed by comparative transcriptomic analysis of sexual and apomictic Paspalum simplex Morong flowers. J Exp Bot 61(6):1869–1883
Chen L, Miyazaki C, Kojimai A, Saito A, Adachi T (1999) Isolation and characterization of a gene expressed during early embryo sac development in apomictic guinea grass (Panicum maximum). J Plant Physiol 154(1):55–62
Yamada-Akiyama H, Akiyama Y, Ebina M, Xu Q, Tsuruta S-i, Yazaki J, Kishimoto N, Kikuchi S, Takahara M, Takamizo T (2009) Analysis of expressed sequence tags in apomictic guineagrass (Panicum maximum). J Plant Physiol 166(7):750–761
Sharbel TF, Voigt M-L, Corral JM, Galla G, Kumlehn J, Klukas C, Schreiber F, Vogel H, Rotter B (2010) Apomictic and sexual ovules of Boechera display heterochronic global gene expression patterns. Plant Cell 22(3):655–671
Sharbel TF, Voigt ML, Corral JM, Thiel T, Varshney A, Kumlehn J, Vogel H, Rotter B (2009) Molecular signatures of apomictic and sexual ovules in the Boechera holboellii complex. Plant J 58(5):870–882
Amiteye S, Corral JM, Vogel H, Sharbel TF (2011) Analysis of conserved microRNAs in floral tissues of sexual and apomictic Boechera species. BMC Genomics 12(1):1
Sahu PP, Gupta S, Malaviya D, Roy AK, Kaushal P, Prasad M (2012) Transcriptome analysis of differentially expressed genes during embryo sac development in apomeiotic non-parthenogenetic interspecific hybrid of Pennisetum glaucum. Mol Biotechnol 51(3):262–271
Albertini E, Marconi G, Barcaccia G, Raggi L, Falcinelli M (2004) Isolation of candidate genes for apomixis in Poa pratensis L. Plant Mol Biol 56(6):879–894
Albertini E, Marconi G, Reale L, Barcaccia G, Porceddu A, Ferranti F, Falcinelli M (2005) SERK and APOSTART. Candidate genes for apomixis in Poa pratensis. Plant Physiol 138(4):2185–2199
Galla G, Vogel H, Sharbel TF, Barcaccia G (2015) De novo sequencing of the Hypericum perforatum L. flower transcriptome to identify potential genes that are related to plant reproduction sensu lato. BMC Genomics 16(1):1
Kumar V, Malik SK, Pal D, Srinivasan R, Bhat SR (2014) Comparative transcriptome analysis of ovules reveals stress related genes associated with nucellar polyembryony in citrus. Tree Genet Genomes 10(3):449–464
Nakano M, Kigoshi K, Shimizu T, Endo T, Shimada T, Fujii H, Omura M (2013) Characterization of genes associated with polyembryony and in vitro somatic embryogenesis in citrus. Tree Genet Genomes 9(3):795–803
Okada T, Hu Y, Tucker MR, Taylor JM, Johnson SD, Spriggs A, Tsuchiya T, Oelkers K, Rodrigues JC, Koltunow AM (2013) Enlarging cells initiating apomixis in Hieracium praealtum transition to an embryo sac program prior to entering mitosis. Plant Physiol 163(1):216–231
Schmidt A, Schmid MW, Klostermeier UC, Qi W, Guthörl D, Sailer C, Waller M, Rosenstiel P, Grossniklaus U (2014) Apomictic and sexual germline development differ with respect to cell cycle, transcriptional, hormonal and epigenetic regulation. PLoS Genet 10(7):e1004476
Corral JM, Vogel H, Aliyu OM, Hensel G, Thiel T, Kumlehn J, Sharbel TF (2013) A conserved apomixis-specific polymorphism is correlated with exclusive exonuclease expression in premeiotic ovules of apomictic Boechera species. Plant Physiol 163(4):1660–1672
Siena LA, Ortiz JPA, Calderini O, Paolocci F, Cáceres ME, Kaushal P, Grisan S, Pessino SC, Pupilli F (2016) An apomixis-linked ORC3-like pseudogene is associated with silencing of its functional homolog in apomictic Paspalum simplex. J Exp Bot 67(6):1965–1978
Conner JA, Goel S, Gunawan G, Cordonnier-Pratt M-M, Johnson VE, Liang C, Wang H, Pratt LH, Mullet JE, DeBarry J (2008) Sequence analysis of bacterial artificial chromosome clones from the apospory-specific genomic region of Pennisetum and Cenchrus. Plant Physiol 147(3):1396–1411
Akiyama Y, Goel S, Conner JA, Hanna WW, Yamada-Akiyama H, Ozias-Akins P (2011) Evolution of the apomixis transmitting chromosome in Pennisetum. BMC Evol Biol 11(1):1
Boutilier K, Offringa R, Sharma VK, Kieft H, Ouellet T, Zhang L, Hattori J, Liu C-M, van Lammeren AA, Miki BL (2002) Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth. Plant Cell 14(8):1737–1749
Kim S, Soltis PS, Wall K, Soltis DE (2006) Phylogeny and domain evolution in the APETALA2-like gene family. Mol Biol Evol 23(1):107–120
El Ouakfaoui S, Schnell J, Abdeen A, Colville A, Labbé H, Han S, Baum B, Laberge S, Miki B (2010) Control of somatic embryogenesis and embryo development by AP2 transcription factors. Plant Mol Biol 74(4–5):313–326
Conner JA, Mookkan M, Huo H, Chae K, Ozias-Akins P (2015) A parthenogenesis gene of apomict origin elicits embryo formation from unfertilized eggs in a sexual plant. Proc Natl Acad Sci 112(36):11205–11210
Lutts S, Ndikumana J, Louant B (1994) Male and female sporogenesis and gametogenesis in apomictic Brachiaria brizantha, Brachiaria decumbens and F1 hybrids with sexual colchicine induced tetraploid Brachiaria ruziziensis. Euphytica 78(1–2):19–25
Swenne A, Louant B, Dujardin M (1981) Induction par la colchicine de formes autotétraploïdes chez Brachiaria ruziziensis Germain et Evrard (Graminée). Agron Trop 36(2):134–141
Pinheiro A, Pozzobon M, Do Valle C, Penteado M, Carneiro V (2000) Duplication of the chromosome number of diploid Brachiaria brizantha plants using colchicine. Plant Cell Rep 19(3):274–278
Simioni C, Cd V (2009) Chromosome duplication in Brachiaria (A. Rich.) Stapf allows intraspecific crosses. Crop Breed Appl Biotechnol 9(4):328–333
Miles JW (2007) Apomixis for cultivar development in tropical forage grasses. Crop Science 47(Suppl 3):S-238-S-249
Singh M, Conner J, Zeng Y-J, Hanna W, Johnson V, Ozias-Akins P (2010) Characterization of apomictic BC7 and BC8 pearl millet: meiotic chromosome behavior and construction of an ASGR-carrier chromosome-specific library. Crop Sci 50(3):892–902
Leblanc O, Grimanelli D, Hernandez-Rodriguez M, Galindo PA, Soriano-Martinez AM, Perotti E (2009) Seed development and inheritance studies in apomictic maize-Tripsacum hybrids reveal barriers for the transfer of apomixis into sexual crops. Int J Dev Biol 53(4):585–596
Ravi M, Marimuthu MP, Siddiqi I (2008) Gamete formation without meiosis in Arabidopsis. Nature 451(7182):1121–1124
Pawlowski WP, Wang C-JR, Golubovskaya IN, Szymaniak JM, Shi L, Hamant O, Zhu T, Harper L, Sheridan WF, Cande WZ (2009) Maize AMEIOTIC1 is essential for multiple early meiotic processes and likely required for the initiation of meiosis. Proc Natl Acad Sci 106(9):3603–3608
Guitton A-E, Berger F (2005) Loss of function of MULTICOPY SUPPRESSOR OF IRA 1 produces nonviable parthenogenetic embryos in Arabidopsis. Curr Biol 15(8):750–754
Rövekamp M, Bowman JL, Grossniklaus U (2016) Marchantia MpRKD regulates the gametophyte-sporophyte transition by keeping egg cells quiescent in the absence of fertilization. Curr Biol 26(13):1782–1789
Grossniklaus U, Vielle-Calzada J-P, Hoeppner MA, Gagliano WB (1998) Maternal control of embryogenesis by MEDEA, a polycomb group gene in Arabidopsis. Science 280(5362):446–450
Kinoshita T, Yadegari R, Harada JJ, Goldberg RB, Fischer RL (1999) Imprinting of the MEDEA polycomb gene in the Arabidopsis endosperm. Plant Cell 11(10):1945–1952
Chaudhury AM, Ming L, Miller C, Craig S, Dennis ES, Peacock WJ (1997) Fertilization-independent seed development in Arabidopsis thaliana. Proc Natl Acad Sci 94(8):4223–4228
Luo M, Bilodeau P, Koltunow A, Dennis ES, Peacock WJ, Chaudhury AM (1999) Genes controlling fertilization-independent seed development in Arabidopsis thaliana. Proc Natl Acad Sci 96(1):296–301
Ohad N, Margossian L, Hsu Y-C, Williams C, Repetti P, Fischer RL (1996) A mutation that allows endosperm development without fertilization. Proc Natl Acad Sci 93(11):5319–5324
Ohad N, Yadegari R, Margossian L, Hannon M, Michaeli D, Harada JJ, Goldberg RB, Fischer RL (1999) Mutations in FIE, a WD polycomb group gene, allow endosperm development without fertilization. Plant Cell 11(3):407–415
Köhler C, Hennig L, Bouveret R, Gheyselinck J, Grossniklaus U, Gruissem W (2003) Arabidopsis MSI1 is a component of the MEA/FIE Polycomb group complex and required for seed development. EMBO J 22(18):4804–4814
Guitton A-E, Page DR, Chambrier P, Lionnet C, Faure J-E, Grossniklaus U, Berger F (2004) Identification of new members of Fertilisation Independent Seed Polycomb Group pathway involved in the control of seed development in Arabidopsis thaliana. Development 131(12):2971–2981
Rodrigues JC, Tucker MR, Johnson SD, Hrmova M, Koltunow AM (2008) Sexual and apomictic seed formation in Hieracium requires the plant polycomb-group gene FERTILIZATION INDEPENDENT ENDOSPERM. Plant Cell 20(9):2372–2386
Tonosaki K, Kinoshita T (2015) Possible roles for polycomb repressive complex 2 in cereal endosperm. Front Plant Sci 6:144
Olmedo-Monfil V, Durán-Figueroa N, Arteaga-Vázquez M, Demesa-Arévalo E, Autran D, Grimanelli D, Slotkin RK, Martienssen RA, Vielle-Calzada J-P (2010) Control of female gamete formation by a small RNA pathway in Arabidopsis. Nature 464(7288):628–632
Hernández-Lagana E, Rodríguez-Leal D, Lúa J, Vielle-Calzada J-P (2016) A multigenic network of ARGONAUTE4 clade members controls early megaspore formation in Arabidopsis. Genetics 116:188151
Lawit SJ, Albertsen MC, Fox T, Gordon-Kamm W, VAN AMM, CALZADA JPV, ROSILLO CB, AREVALO ED, CHAVEZ CG, LAGANA EH (2016) Methods for reproducing plants asexually and compositions thereof. WO 2016/048909 A1
Garcia-Aguilar M, Michaud C, Leblanc O, Grimanelli D (2010) Inactivation of a DNA methylation pathway in maize reproductive organs results in apomixis-like phenotypes. Plant Cell 22(10):3249–3267
Chan SR, Maruthachalam R, Mercier R, Nogue F (2014) Synthetic clonal reproduction through seeds. US20140298507 A1
Lawit SJ (2012) Self-reproducing hybrid plants. US20120266324 A1
d'Erfurth I, Jolivet S, Froger N, Catrice O, Novatchkova M, Mercier R (2009) Turning meiosis into mitosis. PLoS Biol 7(6):e1000124
d'Erfurth I, Cromer L, Jolivet S, Girard C, Horlow C, Sun Y, To JP, Berchowitz LE, Copenhaver GP, Mercier R (2010) The cyclin-A CYCA1; 2/TAM is required for the meiosis I to meiosis II transition and cooperates with OSD1 for the prophase to first meiotic division transition. PLoS Genet 6(6):e1000989
Mieulet D, Jolivet S, Rivard M, Cromer L, Vernet A, Mayonove P, Pereira L, Droc G, Courtois B, Guiderdoni E (2016) Turning rice meiosis into mitosis. Cell Res 26(11):1242–1254
Schiml S, Puchta H (2016) Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas. Plant Methods 12(1):1
Liang G, Zhang H, Lou D, Yu D (2016) Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing. Sci Rep 6:Article number: 21451
Belhaj K, Chaparro-Garcia A, Kamoun S, Patron NJ, Nekrasov V (2015) Editing plant genomes with CRISPR/Cas9. Curr Opin Biotechnol 32:76–84
Warthmann N, Chen H, Ossowski S, Weigel D, Hervé P (2008) Highly specific gene silencing by artificial miRNAs in rice. PLoS One 3(3):e1829
Schwab R, Ossowski S, Riester M, Warthmann N, Weigel D (2006) Highly specific gene silencing by artificial microRNAs in Arabidopsis. Plant Cell 18(5):1121–1133
Ossowski S, Schwab R, Weigel D (2008) Gene silencing in plants using artificial microRNAs and other small RNAs. Plant J 53(4):674–690. doi:10.1111/j.1365-313X.2007.03328.x
Marimuthu MP, Jolivet S, Ravi M, Pereira L, Davda JN, Cromer L, Wang L, Nogué F, Chan SW, Siddiqi I (2011) Synthetic clonal reproduction through seeds. Science 331(6019):876–876
Kelliher T, Starr D, Wang W, McCuiston J, Zhong H, Nuccio ML, Martin B (2016) Maternal haploids are preferentially induced by CENH3-tailswap transgenic complementation in maize. Front Plant Sci 7:414
Lin B-Y (1984) Ploidy barrier to endosperm development in maize. Genetics 107(1):103–115
Singh M, Goel S, Meeley RB, Dantec C, Parrinello H, Michaud C, Leblanc O, Grimanelli D (2011) Production of viable gametes without meiosis in maize deficient for an ARGONAUTE protein. Plant Cell 23(2):443–458
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Conner, J.A., Ozias-Akins, P. (2017). Apomixis: Engineering the Ability to Harness Hybrid Vigor in Crop Plants. In: Schmidt, A. (eds) Plant Germline Development. Methods in Molecular Biology, vol 1669. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7286-9_2
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7286-9_2
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7285-2
Online ISBN: 978-1-4939-7286-9
eBook Packages: Springer Protocols