Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) for the Analysis of DNA Copy Number Variation

  • Chenghang ZongEmail author
Part of the Neuromethods book series (NM, volume 131)


The genomes of even close kindred cells are not identical due to various forms of genomic variations. To discover the uniqueness of each genome, we need to examine the genome at single-cell resolution. Here we describe the recent progress in the development of single-cell whole-genome amplification methods and the state of art for analyzing one of the major forms of genomic variations—copy number of variations (CNVs). Robust detection of CNVs in single cells has allowed successful clinical applications such as prenatal genome screening and diagnosis.

Key words

Single-cell sequencing Single-cell WGA MALBAC MDA Copy number variations 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Baylor College of MedicineHoustonUSA

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