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Brachypodium Genomics pp 43–55Cite as

A Highly Efficient and Reproducible Fusarium spp. Inoculation Method for Brachypodium distachyon

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1667))

Abstract

Fusarium spp. are devastating fungal pathogens which cause significant losses in many cereal crops like wheat, maize, and barley. Genetic improvement of disease resistance requires an improved understanding of defense-associated processes operating in the host in response to an attack by Fusarium spp. Brachypodium distachyon is emerging as a model where host–cereal-infecting pathogen interactions can be studied conveniently. However, this requires developing an efficient infection assay that facilitates quick screening of germplasm (e.g., mutant lines). Here, we provide an efficient and reproducible Fusarium infection assay for Brachypodium. We believe this method will help further develop Brachypodium as a model for genetic improvement of disease resistance in cereals against Fusarium pathogens.

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Acknowledgments

The authors are grateful to Grains Research and Development Corporation (GRDC), Australia for research funding under CSP00155. Anuj Rana is supported by an Endeavour fellowship from the Australian Government and University Grant Commission (UGC), New Delhi for postdoctoral research fellowship. Jonathan Powell is supported by the GRDC through provision of a graduate research scholarship (GRS10532).

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Authors and Affiliations

  1. Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture, 306 Carmody Road, Brisbane, QLD, 4067, Australia

    Anuj Rana, Aneesh Karunakaran, Timothy L. Fitzgerald, Rosalie Sabburg, Jonathan J. Powell & Kemal Kazan

  2. Department of Genetics, University of Delhi, South Campus, New Delhi, 110021, India

    Anuj Rana

  3. School of Agriculture and Food Science, The University of Queensland, Brisbane, QLD, 4072, Australia

    Aneesh Karunakaran & Elizabeth A. B. Aitken

  4. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD, 4072, Australia

    Robert J. Henry, Jonathan J. Powell & Kemal Kazan

Authors
  1. Anuj Rana
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  2. Aneesh Karunakaran
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  3. Timothy L. Fitzgerald
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  4. Rosalie Sabburg
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  5. Elizabeth A. B. Aitken
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  6. Robert J. Henry
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  7. Jonathan J. Powell
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  8. Kemal Kazan
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Corresponding author

Correspondence to Jonathan J. Powell .

Editor information

Editors and Affiliations

  1. Department of Biodiversity and Molecular Ecology, Fondazione Edmund Mach IASMA, Trentino, Italy

    Gaurav Sablok

  2. Sabanci University, Istanbul, Turkey

    Hikmet Budak

  3. Climate Change Cluster (C3), University of Technology Sydney, Sydney, New South Wales, Australia

    Peter J. Ralph

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Rana, A. et al. (2018). A Highly Efficient and Reproducible Fusarium spp. Inoculation Method for Brachypodium distachyon . In: Sablok, G., Budak, H., Ralph, P. (eds) Brachypodium Genomics. Methods in Molecular Biology, vol 1667. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7278-4_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7278-4_4

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7276-0

  • Online ISBN: 978-1-4939-7278-4

  • eBook Packages: Springer Protocols

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