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Single-Molecule Imaging of Escherichia coli Transmembrane Proteins

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Single Molecule Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1665))

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Abstract

Single-molecule imaging in living cells can provide unique information about biological processes. Bacteria offer some particular challenges for single-molecule imaging due to their small size, only slightly larger than the diffraction limit of visible light. Here, we describe how reliable and reproducible single-molecule data can be obtained for a transmembrane protein in the Gram-negative bacterium Escherichia coli by using live-cell fluorescence microscopy. Fluorescent labeling of a protein by genetic fusion, cell culturing, sample preparation, imaging, and data analysis are discussed.

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Author information

Authors and Affiliations

  1. LaserLaB and Department of Physics and Astronomy, Vrije Universiteit, Amsterdam, The Netherlands

    Aravindan Varadarajan & Felix Oswald

  2. LaserLaB and Department of Molecular Cell Biology, Vrije Universiteit, de Boelelaan 1108, 1081HZ, Amsterdam, The Netherlands

    Yves J. M. Bollen

Authors
  1. Aravindan Varadarajan
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  2. Felix Oswald
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  3. Yves J. M. Bollen
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Corresponding author

Correspondence to Yves J. M. Bollen .

Editor information

Editors and Affiliations

  1. Department of Physics & Astronomy, Vrije Universiteit, Amsterdam, The Netherlands

    Erwin J. G. Peterman

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Varadarajan, A., Oswald, F., Bollen, Y.J.M. (2018). Single-Molecule Imaging of Escherichia coli Transmembrane Proteins. In: Peterman, E. (eds) Single Molecule Analysis. Methods in Molecular Biology, vol 1665. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7271-5_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7271-5_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7270-8

  • Online ISBN: 978-1-4939-7271-5

  • eBook Packages: Springer Protocols

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