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Single Molecule Analysis pp 353–366Cite as

Repurposing a Benchtop Centrifuge for High-Throughput Single-Molecule Force Spectroscopy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1665))

Abstract

We present high-throughput single-molecule manipulation using a benchtop centrifuge, overcoming limitations common in other single-molecule approaches such as high cost, low throughput, technical difficulty, and strict infrastructure requirements. An inexpensive and compact Centrifuge Force Microscope (CFM) adapted to a commercial centrifuge enables use by nonspecialists, and integration with DNA nanoswitches facilitates both reliable measurements and repeated molecular interrogation. Here, we provide detailed protocols for constructing the CFM, creating DNA nanoswitch samples, and carrying out single-molecule force measurements.

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Acknowledgement

W.P.W. acknowledges support from the Arnold and Mabel Beckman Foundation through the Beckman Young Investigator Award, US National Institutes of Health NIGMS R21 RGM107907A and R35 GM119537, the Wyss Institute at Harvard University and Boston Children’s Hospital startup funds.

Author information

Authors and Affiliations

  1. Program in Cellular and Molecular Medicine, Boston Children’s Hospital, Boston, MA, 02115, USA

    Darren Yang & Wesley P. Wong

  2. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, 02115, USA

    Darren Yang & Wesley P. Wong

  3. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA

    Darren Yang & Wesley P. Wong

Authors
  1. Darren Yang
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  2. Wesley P. Wong
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Corresponding author

Correspondence to Wesley P. Wong .

Editor information

Editors and Affiliations

  1. Department of Physics & Astronomy, Vrije Universiteit, Amsterdam, The Netherlands

    Erwin J. G. Peterman

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Yang, D., Wong, W.P. (2018). Repurposing a Benchtop Centrifuge for High-Throughput Single-Molecule Force Spectroscopy. In: Peterman, E. (eds) Single Molecule Analysis. Methods in Molecular Biology, vol 1665. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7271-5_19

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  • DOI: https://doi.org/10.1007/978-1-4939-7271-5_19

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7270-8

  • Online ISBN: 978-1-4939-7271-5

  • eBook Packages: Springer Protocols

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