Abstract
Single molecule fluorescence polarization microscopy (smFPM) is a technique that enables to monitor changes in the orientation of a single labeled protein domain. Here we describe a smFPM microscope set-up and protocols to investigate conformational changes associated with the movement of motor proteins along cytoskeletal tracks.
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Acknowledgements
We thank members of the Sosa’s lab A.B.. Asenjo and M. Airo for discussions and proofreading the manuscript. This work was supported by NIH grant R01GM113164.
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Benoit, M.P.M.H., Sosa, H. (2018). Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin–Microtubule Complexes. In: Peterman, E. (eds) Single Molecule Analysis. Methods in Molecular Biology, vol 1665. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7271-5_11
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DOI: https://doi.org/10.1007/978-1-4939-7271-5_11
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