Abstract
Here, we describe the detailed step-by-step protocol for detection of phosphoproteins in two-dimensional difference gel electrophoresis (DIGE) gels. A standard DIGE protocol is combined with subsequent post-staining with phosphospecific fluorescent dye. The combination of these two methods complements DIGE-based proteome profiling by fluorescence detection of phosphoproteins in the same gel providing additional possibility for sensitive and accurate quantification of the differentially regulated phosphoproteins in biological samples.
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Stasyk, T., Huber, L.A. (2018). DIGE-Based Phosphoproteomic Analysis. In: Ohlendieck, K. (eds) Difference Gel Electrophoresis. Methods in Molecular Biology, vol 1664. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7268-5_7
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DOI: https://doi.org/10.1007/978-1-4939-7268-5_7
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7268-5
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