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Enzyme Assay Methods to Validate DIGE Proteomics Data

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Difference Gel Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1664))

Abstract

Enzyme activity assay methods can be used to corroborate the results generated by Difference Gel Electrophoresis (DIGE) proteomic experiments. Two assay methods were chosen to demonstrate how this can be achieved. Assays for determining the activity of superoxide dismutase and NADH dehydrogenase are outlined in detail in this paper. These methods were chosen as examples because they are frequently used in conjunction with DIGE proteomics.

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Authors and Affiliations

  1. Monaghan Biosciences, Tyholland, Co. Monaghan, Ireland

    Andrew Dowd

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  1. Andrew Dowd
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Correspondence to Andrew Dowd .

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Editors and Affiliations

  1. Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland

    Kay Ohlendieck

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Dowd, A. (2018). Enzyme Assay Methods to Validate DIGE Proteomics Data. In: Ohlendieck, K. (eds) Difference Gel Electrophoresis. Methods in Molecular Biology, vol 1664. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7268-5_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7268-5_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7267-8

  • Online ISBN: 978-1-4939-7268-5

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