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Two-Dimensional Gel Electrophoresis and 2D-DIGE

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Difference Gel Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1664))

Abstract

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) continues to be one of the most versatile and widely used techniques to study the proteome of a biological system. In particular, a modified version of 2D-PAGE, two-dimensional difference gel electrophoresis (2D-DIGE), which uses differential labeling of protein samples with up to three fluorescent tags, offers greater sensitivity and reproducibility over conventional 2D-PAGE gels for differential quantitative analysis of protein expression between experimental groups. Both these methods have distinct advantages in the separation and identification of thousands of individual proteins species including protein isoforms and post-translational modifications. This review will discuss the principles of 2D-PAGE and 2D-DIGE including limitations to the methods. 2D-PAGE and 2D-DIGE continue to be popular methods in bioprocessing-related research (particularly on recombinant Chinese hamster ovary cells), which will also be discussed in the review chapter.

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Authors and Affiliations

  1. National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland

    Paula Meleady

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  1. Paula Meleady
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Correspondence to Paula Meleady .

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Editors and Affiliations

  1. Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland

    Kay Ohlendieck

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Meleady, P. (2018). Two-Dimensional Gel Electrophoresis and 2D-DIGE. In: Ohlendieck, K. (eds) Difference Gel Electrophoresis. Methods in Molecular Biology, vol 1664. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7268-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7268-5_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7267-8

  • Online ISBN: 978-1-4939-7268-5

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