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Overexpression of Recombinant Selenoproteins in E. coli

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Selenoproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1661))

Abstract

Expression of selenoproteins necessitates a process of decoding of a UGA codon from termination of translation to insertion of selenocysteine. The mechanisms of this process pose major challenges with regards to recombinant selenoprotein production in E. coli, which however can be overcome especially if the Sec residue is located close to the C-terminal end, as is the case for several naturally found selenoproteins. This chapter summarizes a method to achieve such a production.

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Acknowledgments

The authors acknowledge funding to ESJA from The Swedish Cancer Society, The Swedish Research Council, Swedish Foundation for Strategic Research, Knut and Alice Wallenberg Foundation, and Karolinska Institutet.

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Authors and Affiliations

  1. Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77, Stockholm, Sweden

    Qing Cheng & Elias S. J. Arnér

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  1. Qing Cheng
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  2. Elias S. J. Arnér
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Corresponding author

Correspondence to Elias S. J. Arnér .

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Editors and Affiliations

  1. Centre International de Recherche en Infectiologie, CIRI, Lyon, France

    Laurent Chavatte

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Cheng, Q., Arnér, E.S.J. (2018). Overexpression of Recombinant Selenoproteins in E. coli . In: Chavatte, L. (eds) Selenoproteins. Methods in Molecular Biology, vol 1661. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7258-6_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7258-6_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7257-9

  • Online ISBN: 978-1-4939-7258-6

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