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Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum

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Selenoproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1661))

Abstract

Selenium is an essential element incorporated to different proteins with important biological functions in connection to antioxidant activity, cancer-protective properties, neurodegenerative pathologies, and prevention of effects of diabetes, among others. In addition, selenoamino acids play a basic role in the global equilibrium of key selenium-biomolecules synthesis, including selenoprotein P, selenoalbumin, and glutathione peroxidase. Homeostasis of these selenium-containing biomolecules involves different organs in living organisms including human, and bloodstream is the connection fluid in this process. Therefore, it is very important to have an analytical methodology suitable for selenium proteins and metabolites speciation in serum and plasma samples. For this purpose, a simultaneous speciation method for Se-containing biomolecules in serum/plasma is described on the basis of in series three-dimensional chromatography: size exclusion, affinity, and anion exchange high performance liquid chromatography (3D/SE-AF-AEC-HPLC), using different columns of each type and hyphenation to inductively coupled plasma-(quadrupole) mass spectrometry (ICP-MS). The method allows the quantitative simultaneous analysis of selenoprotein P (SeP), extracellular glutathione peroxidase (eGPx), selenoalbumin (SeAlb), selenite, and selenate in serum (from human and mouse) using species-unspecific isotope dilution (SUID). In addition, a simplified two-dimensional approach (2D/SE-AF-HPLC-SUID-ICP-MS) is described when selenium metabolites are globally analyzed. The method provides detection limits in the range 0.2–1.3 ng of Se g−1 and avoids typical interferences in this matrix from chloride and bromide with a chromatographic runtime less than 35 min.

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Acknowledgments

The authors thanks to Spanish Ministry of Economy and Competitiveness (Grant CTM2015-67902-C2-1-P and Regional Ministry of Economy, Innovation, Science and Employment (Andalusian Government, Spain), Grant P12-FQM-0442. Gema Rodríguez-Moro thanks Spanish Ministry of Economy and Competitiveness for a predoctoral scholarship BES-2013-064501, and Belén Callejón-Leblic thanks the Ministerio de Educación for a predoctoral scholarship FPU13/03615. Finally, the authors are grateful to FEDER (European Community) for financial support, grants number UNHU13-1E-1611 and UNHU15-CE-3140.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Chemistry, University of Huelva, Huelva, Spain

    Belén Callejón-Leblic, Gema Rodríguez-Moro, Tamara García-Barrera & José Luis Gómez-Ariza

  2. Campus of International Excellence A3, University of Huelva, Huelva, 21007, Spain

    Belén Callejón-Leblic, Gema Rodríguez-Moro, Tamara García-Barrera & José Luis Gómez-Ariza

  3. Health and Environment Research Center (CYSMA), University of Huelva, Huelva, Spain

    Belén Callejón-Leblic, Gema Rodríguez-Moro, Tamara García-Barrera & José Luis Gómez-Ariza

Authors
  1. Belén Callejón-Leblic
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  2. Gema Rodríguez-Moro
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  3. Tamara García-Barrera
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  4. José Luis Gómez-Ariza
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Corresponding author

Correspondence to José Luis Gómez-Ariza .

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Editors and Affiliations

  1. Centre International de Recherche en Infectiologie, CIRI, Lyon, France

    Laurent Chavatte

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Callejón-Leblic, B., Rodríguez-Moro, G., García-Barrera, T., Gómez-Ariza, J.L. (2018). Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum. In: Chavatte, L. (eds) Selenoproteins. Methods in Molecular Biology, vol 1661. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7258-6_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7258-6_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7257-9

  • Online ISBN: 978-1-4939-7258-6

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