Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification
Exosomes were originally described 29 years ago as a mechanism for the removal of redundant molecules from reticulocytes, nothing but a process of removing cellular trash. It is now however, abundantly clear that exosomes have a more significant biological role. However, there is currently limited information pertaining to efficient isolation procedures that can be used to isolate exosomes from both cell culture media, and clinical samples such as plasma. Here, we present a reliable and efficient procedure that can be utilized for the isolation of exosomes from various starting materials.
Key wordsExosomes Extracellular vesicles Isolation Human plasma Cell culture supernatant
This work was supported by a National Health and Medical Research Council Australia project grant (NHMRC, APP1068510) and Cancer Council Queensland grant (APP1045620) to AM.
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