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Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification

  • Richard Lobb
  • Andreas MöllerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1660)

Abstract

Exosomes were originally described 29 years ago as a mechanism for the removal of redundant molecules from reticulocytes, nothing but a process of removing cellular trash. It is now however, abundantly clear that exosomes have a more significant biological role. However, there is currently limited information pertaining to efficient isolation procedures that can be used to isolate exosomes from both cell culture media, and clinical samples such as plasma. Here, we present a reliable and efficient procedure that can be utilized for the isolation of exosomes from various starting materials.

Key words

Exosomes Extracellular vesicles Isolation Human plasma Cell culture supernatant 

Notes

Acknowledgments

This work was supported by a National Health and Medical Research Council Australia project grant (NHMRC, APP1068510) and Cancer Council Queensland grant (APP1045620) to AM.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Tumour Microenvironment LaboratoryQIMR Berghofer Medical Research InstituteHerstonAustralia
  2. 2.School of MedicineUniversity of QueenslandBrisbaneAustralia

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