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Extracellular Vesicles pp 55–63Cite as

Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1660))

Abstract

Extracellular vesicles (EVs) are a heterogeneous group of membrane-encapsulated particles with different ranges of size, density, and cargo. Various types of RNA including mRNA are enclosed within EVs and can serve as novel biomarkers for disease detection and patient management. Ultracentrifugation, precipitation , antibody-based capture and filter-based methods are available as in-house laboratory procedures or commercially available kits to isolate EVs. Here, we describe a filter-based method for EV mRNA isolation that is designed for parallel processing of large sample numbers.

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Author information

Authors and Affiliations

  1. Hitachi Chemical Co. America, Ltd. R & D Center, 1003 Health Sciences Road, Irvine, CA, 92617, USA

    Cindy M. Yamamoto & Taku Murakami

  2. Gynecologic Oncology Division, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, 02115, USA

    Shu-Wing Ng

Authors
  1. Cindy M. Yamamoto
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  2. Taku Murakami
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  3. Shu-Wing Ng
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Corresponding author

Correspondence to Cindy M. Yamamoto .

Editor information

Editors and Affiliations

  1. CloudHealth Genomics, Ltd., Shanghai, China

    Winston Patrick Kuo

  2. Predicine, Inc., Hayward, California, USA

    Shidong Jia

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Yamamoto, C.M., Murakami, T., Ng, SW. (2017). Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis. In: Kuo, W., Jia, S. (eds) Extracellular Vesicles. Methods in Molecular Biology, vol 1660. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7253-1_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7253-1_6

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7251-7

  • Online ISBN: 978-1-4939-7253-1

  • eBook Packages: Springer Protocols

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