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Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis

  • Cindy M. YamamotoEmail author
  • Taku Murakami
  • Shu-Wing Ng
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1660)

Abstract

Extracellular vesicles (EVs) are a heterogeneous group of membrane-encapsulated particles with different ranges of size, density, and cargo. Various types of RNA including mRNA are enclosed within EVs and can serve as novel biomarkers for disease detection and patient management. Ultracentrifugation, precipitation, antibody-based capture and filter-based methods are available as in-house laboratory procedures or commercially available kits to isolate EVs. Here, we describe a filter-based method for EV mRNA isolation that is designed for parallel processing of large sample numbers.

Key words

Exosomes Microvesicles Urine Ascites Extracellular vesicles Filter mRNA Isolation High-throughput Gene expression 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Cindy M. Yamamoto
    • 1
    Email author
  • Taku Murakami
    • 1
  • Shu-Wing Ng
    • 2
  1. 1.Hitachi Chemical Co. America, Ltd. R & D CenterIrvineUSA
  2. 2.Gynecologic Oncology Division, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women’s HospitalHarvard Medical SchoolBostonUSA

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