Therapeutic Use of Tumor Cell-Derived Extracellular Vesicles

  • Jing Liu
  • Jingwei Ma
  • Ke Tang
  • Bo HuangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1660)


Extracellular vesicles (EVs) are nanosized membrane vesicles, released by many cell types, which are an ideal drug-delivery system for antitumor therapy. Exosomes, microparticles (MP), and apoptotic bodies are the three main types of EVs. MPs are EVs with size ranges between 100 and 1000 nm. Tumor cell-derived EVs (T-EVs) can serve as a safe and efficient vehicle to specific deliver chemotherapeutic drugs or oncolytic adenoviruses for tumor treatment. In this section, we describe how to prepare EVs for therapeutic use.

Key words

Extracellular vesicles Tumor microparticles Drug delivery Chemotherapeutic drugs Oncolytic adenoviruses Tumor vaccines Tumor antigens Dendritic cells 



This work was supported by National Basic Research Program of China (2015CB932600), National Science Fund for Distinguished Young Scholars of China (81225021), and National Natural Science Foundation of China (81472735, 81472653, and 81530080). J. Liu and J. Ma contributed equally to this work.


  1. 1.
    Lemoinne S, Thabut D, Housset C, Moreau R, Valla D, Boulanger CM et al (2014) The emerging roles of microvesicles in liver diseases. Nat Rev Gastroenterol Hepatol 11:350–361CrossRefPubMedGoogle Scholar
  2. 2.
    Raposo G, Stoorvogel W (2013) Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol 200:373–383CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    van Dommelen SM, Vader P, Lakhal S, Kooijmans SA, van Solinge WW, Wood MJ, Schiffelers RM (2012) Microvesicles and exosomes: opportunities for cell derived membrane vesicles in drug delivery. J Control Release 161:635–644CrossRefPubMedGoogle Scholar
  4. 4.
    Skog J, Wurdinger T, van Rijn S, Meijer DH, Gainche L, Sena-Esteves M et al (2008) Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat Cell Biol 10:1470–1476CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Zitvogel L, Regnault A, Lozier A, Wolfers J, Flament C, Tenza D et al (1998) Eradication of established murine tumors using a novel cell-free vaccine: dendritic cell derived exosomes. Nat Med 4:594–600CrossRefPubMedGoogle Scholar
  6. 6.
    Dai S, Wei D, Wu Z, Zhou X, Wei X, Huang H et al (2008) Phase I clinical trial of autologous ascites-derived exosomes combined with GM-CSF for colorectal cancer. Mol Ther 16:782–790CrossRefPubMedGoogle Scholar
  7. 7.
    Tang K, Zhang Y, Zhang H, Xu P, Liu J, Ma J et al (2012) Delivery of chemotherapeutic drugs in tumour cell-derived microparticles. Nat Commun 3:1–11Google Scholar
  8. 8.
    Ma J, Zhang Y, Tang K, Zhang H, Yin X, Li Y et al (2016) Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles. Cell Res 26:713–727CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Ran L, Tan X, Li Y, Zhang H, Ma R, Ji T et al (2016) Delivery of oncolytic adenovirus into the nucleus of tumorigenic cells by tumor microparticles for virotherapy. Biomaterials 89:56–66CrossRefPubMedGoogle Scholar
  10. 10.
    Zhang H, Tang K, Zhang Y, Ma R, Ma J, Li Y et al (2015) Cell-free tumor microparticle vaccines stimulate dendritic cells via cGAS/STING signaling. Cancer Immunol Res 3:196–205CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of GastroenterologyZhongnan Hospital of Wuhan UniversityWuhanChina
  2. 2.The Hubei Clinical Center & Key Laboratory of Bowel DiseasesWuhanChina
  3. 3.Biochemistry and Molecular Biology, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  4. 4.Department of Immunology, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  5. 5.State Key Laboratory of Medical Molecular Biology and Department of Immunology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

Personalised recommendations