The Use of Peripheral Extracellular Vesicles for Identification of Molecular Biomarkers in a Solid Tumor Mouse Model
Extracellular vesicles (EVs) have been increasingly recognized as a potential source of disease biomarkers, since they contain a multitude of biologically active protein, DNA and RNA species, and they can be retrieved from circulating blood of patients. Here, we describe a protocol for DNA extraction from exosomes, shedding microvesicles and apoptotic bodies isolated from peripheral blood in a mouse xenograft model of solid tumor. In this model, human DNA isolated from tumor-derived EVs can be readily distinguished from the one of the hosts, which is of particular interest for studies aimed at molecular characterization of tumor biomarkers.
Key wordsExtracellular vesicles Glioblastoma Xenograft Biomarkers Peripheral blood
This work was supported in part by grants from Health Research Fund (Fondo de Investigaciones Sanitarias, FIS), Carlos III Health Institute (PI10/01069, CP11/00147, and PI14/00077), Plan Estatal I + D + I 2013–2016, and Ministerio de Economía y Competitividad (RTC-2015-3846-1) (A.A.S.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Noemí García-Romero and Gorjana Rackov contributed equally to this work.
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