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Electric Field-Induced Disruption and Releasing Viable Content from Extracellular Vesicles

  • Chris Wang
  • Austin Wang
  • Fang Wei
  • David T. W. Wong
  • Michael TuEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1660)

Abstract

In order to more fully elucidate the biogenesis of exosomes, a type of extracellular vesicles (EVs) and understand the role of EVs in disease processes, it is necessary to develop methods to capture EVs and induce the unloading of viable cargo. Traditionally, ultracentrifugation followed by chemical based EV lysis techniques is used to isolate these extracellular vesicles and release their internal content. Here, we describe a novel technique for capturing and releasing exosomal content through magnetic bead-based EV extraction coupled with electric field induced release and measurement (EFIRM). The usage of low-voltage electric fields allows the EV to be lysed without chemical treatment, and surface immobilized probes can allow for the rapid capture of the content of lysed EVs. EFIRM as an integrated EV lysing and analysis system offers great potential for the investigation of EVs in clinical and basic science contexts.

Key words

Exosomes Extracellular vesicles Electric field induced release and measurement (EFIRM) Biomarkers Diagnostics 

Notes

Acknowledgments

Research reported in this publication was supported by the National Institute of Dental & Craniofacial Research of the National Institutes of Health under Award Number T90DE022734. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Disclosures: David Wong is cofounder of RNAmeTRIX Inc., a molecular diagnostic company. He holds equity in RNAmeTRIX, and serves as a company Director and Scientific Advisor. The University of California also holds equity in RNAmeTRIX. Intellectual property that David Wong invented and which was patented by the University of California and has been licensed to RNAmeTRIX. Additionally, he is a consultant to PeriRx.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Chris Wang
    • 1
  • Austin Wang
    • 1
  • Fang Wei
    • 1
  • David T. W. Wong
    • 1
  • Michael Tu
    • 1
    Email author
  1. 1.School of DentistryUniversity of CaliforniaLos AngelesUSA

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