Isolation of Extracellular Vesicles from Breast Milk

  • Xin WangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1660)


Isolation of extracellular vesicles (EVs) from breast milk enables the downstream analysis of the contents, including various proteins, lipids, and RNAs (messenger RNAs and microRNAs). Here, we present the method of centrifugation with ExoQuick™ precipitation to isolate EVs from breast milk. It has been proven to be a rapid and effective alternative compared to conventional differential centrifugation method.

Key words

Breast milk Extracellular vesicles Centrifugation ExoQuick™ precipitation 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Center for Biomimetic MedicineHouston Methodist Research InstituteHoustonUSA

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