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Extracellular Vesicle miRNA Detection Using Molecular Beacons

  • Won Jong RheeEmail author
  • Seunga Jeong
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1660)

Abstract

Many studies have recently focused on extracellular vesicles. Extracellular vesicles including exosomes are nanosized vesicles that are produced and secreted by cells. Extracellular vesicles contain various types of nucleic acid biomarkers, including microRNAs (miRNAs) from their parent cells. As high concentrations of extracellular vesicles are found in most body fluids, miRNA detection of exosomes in body fluids, including blood and urine, enables early and easy disease diagnosis. In this context, the in situ detection method of extracellular vesicles miRNA using molecular beacons is described here.

Key words

Extracellular vesicle Exosome miRNA Diagnosis Molecular beacon 

Notes

Acknowledgment

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2014R1A1A2054839).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Division of BioengineeringIncheon National UniversityIncheonSouth Korea

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