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Extracellular Vesicle miRNA Detection Using Molecular Beacons

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Extracellular Vesicles

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1660))

Abstract

Many studies have recently focused on extracellular vesicles. Extracellular vesicles including exosomes are nanosized vesicles that are produced and secreted by cells. Extracellular vesicles contain various types of nucleic acid biomarkers, including microRNAs (miRNAs ) from their parent cells. As high concentrations of extracellular vesicles are found in most body fluids, miRNA detection of exosomes in body fluids, including blood and urine, enables early and easy disease diagnosis. In this context, the in situ detection method of extracellular vesicles miRNA using molecular beacons is described here.

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Acknowledgment

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2014R1A1A2054839).

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Authors and Affiliations

  1. Division of Bioengineering, Incheon National University, Songdo-dong, 119 Academy-ro, Yeonsu-gu, Incheon, 406-772, South Korea

    Won Jong Rhee & Seunga Jeong

Authors
  1. Won Jong Rhee
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  2. Seunga Jeong
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Corresponding author

Correspondence to Won Jong Rhee .

Editor information

Editors and Affiliations

  1. CloudHealth Genomics, Ltd., Shanghai, China

    Winston Patrick Kuo

  2. Predicine, Inc., Hayward, California, USA

    Shidong Jia

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Rhee, W.J., Jeong, S. (2017). Extracellular Vesicle miRNA Detection Using Molecular Beacons. In: Kuo, W., Jia, S. (eds) Extracellular Vesicles. Methods in Molecular Biology, vol 1660. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7253-1_23

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  • DOI: https://doi.org/10.1007/978-1-4939-7253-1_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7251-7

  • Online ISBN: 978-1-4939-7253-1

  • eBook Packages: Springer Protocols

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